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DNA Research Advance Access published online on January 14, 2009
DNA Research, doi:10.1093/dnares/dsn032
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© The Author 2009. Kazusa DNA Research Institute
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Periodic Gene Expression Patterns during the Highly Synchronized Cell Nucleus and Organelle Division Cycles in the Unicellular Red Alga Cyanidioschyzon merolae

Takayuki Fujiwara1, Osami Misumi1, Kousuke Tashiro2, Yamato Yoshida3, Keiji Nishida1, Fumi Yagisawa1, Sousuke Imamura4, Masaki Yoshida1, Toshiyuki Mori5, Kan Tanaka4,6, Haruko Kuroiwa1 and Tsuneyoshi Kuroiwa1,*

1 Research Information Center for Extremophile, Rikkyo University, 3-34-1 Nishiikebukuro, Toshima, Tokyo 171-8501, Japan
2 Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan
3 Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5, Kashiwanoha, Kashiwa, Chiba 277-8562, Japan
4 Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
5 Miyagishima Initiative Research Unit, RIKEN Frontier Research System, 2-1, Hirosawa, Wako, Saitama 351-0198, Japan
6 Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba 271-8510, Japan

Received 31 October 2008 ; accepted 2 December 2008.

Previous cell cycle studies have been based on cell-nuclear proliferation only. Eukaryotic cells, however, have double membranes-bound organelles, such as the cell nucleus, mitochondrion, plastids and single-membrane-bound organelles such as ER, the Golgi body, vacuoles (lysosomes) and microbodies. Organelle proliferations, which are very important for cell functions, are poorly understood. To clarify this, we performed a microarray analysis during the cell cycle of Cyanidioschyzon merolae. C. merolae cells contain a minimum set of organelles that divide synchronously. The nuclear, mitochondrial and plastid genomes were completely sequenced. The results showed that, of 158 genes induced during the S or G2-M phase, 93 were known and contained genes related to mitochondrial division, ftsZ1-1, ftsz1-2 and mda1, and plastid division, ftsZ2-1, ftsZ2-2 and cmdnm2. Moreover, three genes, involved in vesicle trafficking between the single-membrane organelles such as vps29 and the Rab family protein, were identified and might be related to partitioning of single-membrane-bound organelles. In other genes, 46 were hypothetical and 19 were hypothetical conserved. The possibility of finding novel organelle division genes from hypothetical and hypothetical conserved genes in the S and G2-M expression groups is discussed.

Key words: cell cycle; microarray; mitochondria–plastid division genes; organelle division genes; Cyanidioschyzon merolae

* To whom correspondence should be addressed. Tel. +81-3-3985-4592. Fax: +81-3-3985-4592. E-mail: tsune{at}rikkyo.ne.jp

Edited by Katsumi Isono


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[Abstract] [Full Text] [PDF]


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