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DNA Research Advance Access published online on September 14, 2008
DNA Research, doi:10.1093/dnares/dsn022
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© The Author 2008. Kazusa DNA Research Institute
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

A High-density Linkage Map of Lotus japonicus Based on AFLP and SSR Markers

Xinwang Wang1,* {dagger}, Shusei Sato2, Satoshi Tabata2 and Shinji Kawasaki1,*

1 National Institute of Agrobiological Sciences, Kannon-dai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan
2 Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan

Received 18 June 2008 ; accepted 18 August 2008.

A collection of 94 F6 individuals derived from crosses between Lotus japonicus, Gifu B-129 (G) and Miyakojima MG-20 (M) were used for mapping. By using the HEGS running system, 427 EcoRI/MseI primer pairs were selected to generate a total of 2053 markers, consisting of 739 G-associated dominant markers, 674 M-associated dominant markers, 640 co-dominant markers, 95 SSR markers and 2 dCAPS markers. Excluding heavily distorted markers, 1588 were mapped to six chromosomes of the L. japonicus genome based on the 97 reference markers. This linkage map consisted of 1023 unique markers (excluding duplicated markers) and covered a total of 508.5 cM of the genome with an average chromosome length of 84.7 cM and interval distance of 0.50 cM. Fifteen quantitative traits loci for eight morphological traits were also mapped. This linkage map will provide a useful framework for physical map construction in L. japonicus in the near future.

Key words: Lotus japonicus; AFLP; SSR; linkage map; HEGS (high efficiency genome scanning)

* To whom correspondence should be addressed. Tel. +1 865-974-1070. Fax. +1 865-974-4744. E-mail: xinwangwang{at}hotmail.com (X.W.) or kawasa{at}nias.affrc.go.jp (S.K.)

Edited by Katsumi Isono

{dagger} Present address: The University of Tennessee, Knoxville, TN 37996, USA.


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