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DNA Research Advance Access published online on November 13, 2007
DNA Research, doi:10.1093/dnares/dsm021
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© The Author 2007. Kazusa DNA Research Institute.
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

A Large-scale Protein–protein Interaction Analysis in Synechocystis sp. PCC6803

Shusei Sato*, Yoshikazu Shimoda, Akiko Muraki, Mitsuyo Kohara, Yasukazu Nakamura and Satoshi Tabata

Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan

Received 5 September 2007 ; accepted 9 October 2007.

Protein–protein interactions (PPIs) play crucial roles in protein function for a variety of biological processes. Data from large-scale PPI screening has contributed to understanding the function of a large number of predicted genes from fully sequenced genomes. Here, we report the systematic identification of protein interactions for the unicellular cyanobacterium Synechocystis sp. strain PCC6803. Using a modified high-throughput yeast two-hybrid assay, we screened 1825 genes selected primarily from (i) genes of two-component signal transducers of Synechocystis, (ii) Synechocystis genes whose homologues are conserved in the genome of Arabidopsis thaliana, and (iii) genes of unknown function on the Synechocystis chromosome. A total of 3236 independent two-hybrid interactions involving 1920 proteins (52% of the total protein coding genes) were identified and each interaction was evaluated using an interaction generality (IG) measure, as well as the general features of interacting partners. The interaction data obtained in this study should provide new insights and novel strategies for functional analyses of genes in Synechocystis, and, additionally, genes in other cyanobacteria and plant genes of cyanobacterial origin.

Key words: Synechocystis sp. strain PCC6803; protein–protein interactions; yeast two-hybrid system

* To whom correspondence should be addressed. Tel. +81-438-52-3935. Fax. +81-438-52-3934. E-mail: ssato{at}kazusa.or.jp

Edited by Takashi Ito


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