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DNA Research Advance Access published online on June 25, 2007
DNA Research, doi:10.1093/dnares/dsm012
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© The Author 2007. Kazusa DNA Research Institute
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Short Communication

Radiation Hybrid Maps of Medaka Chromosomes LG 12, 17, and 22

Feng Su1, Yumi Osada2, Marc Ekker3, Mario Chevrette4, Atsushi Shimizu5, Shuichi Asakawa5, Aiko Shiohama5, Takashi Sasaki5, Nobuyoshi Shimizu5, Toshiyuki Yamanaka2, Takao Sasado2, Hiroshi Mitani6, Robert Geisler7, Hisato Kondoh1,2 and Makoto Furutani-Seiki2,* {dagger}

1 The Graduate School of Frontier Biosciences, Osaka University, 1–3 Yamadaoka, Suita, Osaka 565-0871, Japan
2 SORST Kondoh Research Team, Japan Science and Technology Agency (JST), 14 Yoshida-Kawaracho, Sakyo-ku, Kyoto 606-8305, Japan
3 Department of Biology, Center for Advanced Research in Environmental Genomics, University of Ottawa, 20, Marie Curie, Ottawa, ON, Canada K1N 6N5
4 The Research Institute of the McGill University Health Centre and Department of Surgery, McGill University, Montreal, QC, Canada H3G 1A4
5 Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan
6 Department of Integrated Bioscience, Graduate School of Frontier Science, The University of Tokyo, Bioscience Building, 102, Kashiwa, Chiba 277-8562, Japan
7 Max-Planck-Institut für Entwicklungsbiologie, Abteilung III–Genetik, Spemannstrasse 35, Tübingen D-72076, Germany

Received 15 December 2006 ; revised 8 May 2007

The Medaka is an excellent genetic system for studies of vertebrate development and disease and environmental and evolutionary biology studies. To facilitate the mapping of markers or the cloning of affected genes in Medaka mutants identified by forward-genetic screens, we have established a panel of whole-genome radiation hybrids (RHs) and RH maps for three Medaka chromosomes. RH mapping is useful, since markers to be mapped need not be polymorphic and one can establish the order of markers that are difficult to resolve by genetic mapping owing to low genetic recombination rates. RHs were generated by fusing the irradiated donor, OLF-136 Medaka cell line, with the host B78 mouse melanoma cells. Of 290 initial RH clones, we selected 93 on the basis of high retention of fragments of the Medaka genome to establish a panel that allows genotyping in the 96-well format. RH maps for linkage groups 12, 17, and 22 were generated using 159 markers. The average retention for the three chromosomes was 19% and the average break point frequency was ~33 kb/cR. We estimate the potential resolution of the RH panel to be ~186 kb, which is high enough for integrating RH data with bacterial artificial chromosome clones. Thus, this first RH panel will be a useful tool for mapping mutated genes in Medaka.

Key words: Medaka; radiation hybrid mapping; genetic mapping; BAC

* To whom correspondence should be addressed. Tel. +44 (0) 1225 38 5046. Fax. +44 (0) 1225 38 6779. E-mail: furutaniseiki{at}msi.biglobe.ne.jp

Communicated by Yuji Kohara

{dagger} Present address: Centre for Regenerative Medicine, Developmental Biology Programme, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK


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