DNA Research Advance Access published online on May 31, 2007
DNA Research, doi:10.1093/dnares/dsm008
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Analysis of Expressed Sequence Tags from the Fungus Aspergillus oryzae Cultured Under Different Conditions
1 National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan
2 Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
3 National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
4 Department of Biotechnology, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
5 Higeta Shoyu Co., Ltd, 2-8 Chuo-cho, Choshi, Chiba 288-8680, Japan
6 Gekkeikan Sake Co., Ltd, 101 Shimotoba-koyanagi-cho, Fushimi-ku, Kyoto, 612-8385, Japan
7 Kikkoman Corporation, 250 Noda, Noda-city, Chiba 278-8601, Japan
8 Amano Enzyme Inc., 4-179-35 Sue-cho, Kakamigahara, Gifu 509-0108, Japan
9 Food Research Center, Aichi Industrial Technology Institute, 2-1-1, Shinpukuji-cho, Nishi-ku, Nagoya, Aichi 451-0083 Japan
10 Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi 981-8555, Japan
11 Division of Agriscience and Bioscience, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan
12 Department of Biological Mechanisms and Functions, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan
Received 20 February 2007 ; revised 8 April 2007
We performed random sequencing of cDNAs from nine biologically or industrially important cultures of the industrially valuable fungus Aspergillus oryzae to obtain expressed sequence tags (ESTs). Consequently, 21 446 raw ESTs were accumulated and subsequently assembled to 7589 non-redundant consensus sequences (contigs). Among all contigs, 5491 (72.4%) were derived from only a particular culture. These included 4735 (62.4%) singletons, i.e. lone ESTs overlapping with no others. These data showed that consideration of culture grown under various conditions as cDNA sources enabled efficient collection of ESTs. BLAST searches against the public databases showed that 2953 (38.9%) of the EST contigs showed significant similarities to deposited sequences with known functions, 793 (10.5%) were similar to hypothetical proteins, and the remaining 3843 (50.6%) showed no significant similarity to sequences in the databases. Culture-specific contigs were extracted on the basis of the EST frequency normalized by the total number for each culture condition. In addition, contig sequences were compared with sequence sets in eukaryotic orthologous groups (KOGs), and classified into the KOG functional categories.
Key words: Aspergillus oryzae; cDNA; EST; solid-state culture; liquid culture
* To whom correspondence should be addressed. Tel. +81-82-420-0826, Fax. +81-82-420-0809, E-mail: akao_t{at}nrib.go.jp
Communicated by Naotake Ogasawara
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