DNA Research Advance Access published online on October 17, 2006
DNA Research, doi:10.1093/dnares/dsl010
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
* To whom correspondence should be addressed. Nitrogen starvation requires cells to change their transcriptome in order to cope with this essential nutrient limitation. Here, using microarray analysis, we investigated changes in transcript profiles following nitrogen depletion in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Results revealed that genes for sugar catabolic pathways including glycolysis, oxidative pentose phosphate (OPP) pathway, and glycogen catabolism were induced by nitrogen depletion, and activities of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD), two key enzymes of the OPP pathway, were demonstrated to increase under this condition. We recently showed that a group 2 sigma factor SigE, which is under the control of the global nitrogen regulator NtcA, positively regulated these sugar catabolic pathways. However, increases of transcript levels of these sugar catabolic genes under nitrogen starvation were still observed even in a sigE-deficient mutant, indicating the involvement of other regulatory element(s) in addition to SigE. Since these nitrogen activations were abolished in an ntcA mutant, and since these genes were not directly included in the NtcA regulon, we suggested that sugar catabolic genes were induced by nitrogen depletion under complex and redundant regulations including SigE and other unknown factor(s) under the control of NtcA.
Received June 7, 2006
Revised September 18, 2006
Full Papers
Nitrogen Induction of Sugar Catabolic Gene Expression in Synechocystis sp. PCC 6803
Takashi Osanai 1, Sousuke Imamura 2, Munehiko Asayama 3, Makoto Shirai 3, Iwane Suzuki 4, Norio Murata 4, and Kan Tanaka 1 *
2 Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan; Laboratory of Molecular Genetics, College of Agriculture, Ibaraki University, Ami, Inashiki, Ibaraki 300-0393, Japan
3 Laboratory of Molecular Genetics, College of Agriculture, Ibaraki University, Ami, Inashiki, Ibaraki 300-0393, Japan
4 Department for Regulation Biology, National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan
Kan Tanaka, E-mail: kntanaka{at}iam.u-tokyo.ac.jp
Abstract
Communicated by Satoshi Tabata
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Asayama and S. Imamura Stringent promoter recognition and autoregulation by the group 3 {sigma}-factor SigF in the cyanobacterium Synechocystis sp. strain PCC 6803 Nucleic Acids Res., August 8, 2008; (2008) gkn453v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Vavilin, D. Yao, and W. Vermaas Small Cab-like Proteins Retard Degradation of Photosystem II-associated Chlorophyll in Synechocystis sp. PCC 6803: KINETIC ANALYSIS OF PIGMENT LABELING WITH 15N AND 13C J. Biol. Chem., December 28, 2007; 282(52): 37660 - 37668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Osanai and K. Tanaka Keeping in Touch with PII: PII-Interacting Proteins in Unicellular Cyanobacteria Plant Cell Physiol., July 1, 2007; 48(7): 908 - 914. [Abstract] [Full Text] [PDF]
|
|