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DNA Research Advance Access published online on September 16, 2006
DNA Research, doi:10.1093/dnares/dsl002
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© The Author 2006. Kazusa DNA Research Institute.
Received May 23, 2006
Revised July 12, 2006

Full Papers

Genomic Instability of the DYZ1 Repeat in Patients with Y Chromosome Anomalies and Males Exposed to Natural Background Radiation

Deepali Pathak 1, Sanjay Premi 1, Jyoti Srivastava 1, Sebastian Padinjarel Chandy 1, and Sher Ali 1 *

1 Molecular Genetics Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 11 0067, India

* To whom correspondence should be addressed.
Sher Ali, E-mail: sheralib5{at}hotmail.com


  Abstract

We assessed genomic instability of 3.4 kb DYZ1 repeat arrays in patients encompassing prostate cancer (PC), cases of repeated abortion (RA) and males exposed to natural background radiation (NBR) using real-time PCR and fluorescence in situ hybridization (FISH). Normal males showed DYZ1 copies ranging from 3000 to 4300, RA, 0-2237; PC, 550; and males exposed to NBR, 1577-5700. FISH showed organizational variation of DYZ1 in these samples substantiating the data obtained from real-time PCR. Of the 10 RA samples, 7 were found to be affected of which, 5 showed deletion of 265 bp from nt 25 to 290 and 773 bp from 1347 to 2119 and 2 showed deletion of 275 bp from nt 3128 to 3402. Copy number variation of DYZ1 in these males correlated with genetic constrains/anomalies. Although precise mechanisms of genomic instability of DYZ1 remains unclear, we construe that this repeat plays a critical role in maintaining the structural integrity of the Y chromosome, possibly by absorbing the load of mutations. This may be used as a marker system to analyze genetic integrity of the DYZ1 repeat array(s) across the spectrum of patients.

Keywords: copy number variation; DYZ1 repeat fraction; Y chromosome dysfunction; repeated abortion; prostate cancer.
Communicated by Minoru Ko
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