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DNA Research Advance Access published online on March 29, 2006
DNA Research, doi:10.1093/dnares/dsi030
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© The Author 2006. Kazusa DNA Research Institute.
Received December 1, 2005
Revised February 21, 2006

Article

Genome-wide Searching of Single-nucleotide Polymorphisms among Eight Distantly and Closely Related Rice Cultivars (Oryza sativa L.) and a Wild Accession (Oryza rufipogon Griff.)

Lisa Monna 1 *, Rieko Ohta 1, Haruka Masuda 1, Akiko Koike 1, and Yuzo Minobe 1

1 Plant Genome Center, 1-25-2 Kan-nondai, Tsukuba, Ibaraki 305-0856, Japan

* To whom correspondence should be addressed.
Lisa Monna, E-mail: monna{at}pgcdna.co.jp


  Abstract

We searched the genomes of eight rice cultivars (Oryza sativa L. ssp. japonica and ssp. indica) and a wild rice accession (Oryza rufipogon Griffith) for nucleotide polymorphisms, and identified 7805 polymorphic loci, including single-nucleotide polymorphisms (SNPs) and insertions/deletions (InDels), in predicted intergenic regions. Polymorphisms are useful as DNA markers for genetic analysis or positional cloning with segregating populations of crosses. Pairwise comparison between cultivars and a neighbor-joining tree calculated from SNPs agreed very well with relationships between rice strains predicted from pedigree data or calculated with other DNA markers such as p-SINE1 and simple sequence repeats (SSRs), suggesting that whole-genome SNP information can be used for analysis of evolutionary relationships. Using multiple SNPs to identify alleles, we drew a map to illustrate the alleles shared among the eight cultivars and the accession. The map revealed that most of the genome is mono- or di-allelic among japonica cultivars, whereas alleles well conserved among modern japonica paddy rice cultivars were often shared with indica cultivars or wild rice, suggesting that the genome structure of modern cultivars is composed of chromosomal segments from various genetic backgrounds. Use of allele-sharing analysis and association analysis were also tested and are discussed.

Keywords: sequence; variety-specific; allele; DNA marker; wild rice.
Communicated by Masahiro Yano
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