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DNA Research Advance Access published online on February 22, 2006
DNA Research, doi:10.1093/dnares/dsi025
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© The Author 2006. Kazusa DNA Research Institute.
Received August 26, 2005
Revised December 19, 2005

Full Papers

Identification of Thirty-four Transcripts Expressed Specifically in Hemocytes of Ciona intestinalis and Their Expression Profiles throughout the Life Cycle

Michio Ogasawara 1, Noriko Nakazawa 1, Kaoru Azumi 2, Eiji Yamabe 3, Nori Satoh 4, and Masanobu Satake 3 *

1 Department of Biology, Faculty of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan
2 Division of Innovative Research, Creative Research Initiative "Sousei’, Hokkaido University Sapporo 001-0021, Japan
3 Institute of Development, Aging and Cancer, Tohoku University, Seiryo-machi 4-1, Aoba-ku, Sendai 980-8575, Japan
4 Department of Zoology, Graduate School of Science, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan

* To whom correspondence should be addressed.
Masanobu Satake, E-mail: satake{at}idac.tohoku.ac.jp


  Abstract

The innate immunity of ascidian hemocytes is considered to be a prototype of that in vertebrates. In this study, we identified as many transcripts as possible that were expressed specifically in hemocytes of Ciona intestinalis, a ubiquitous species of ascidian. Using a large-scale whole-mount in situ hybridization (WISH) technique and young adult specimens of C. intestinalis, 34 such transcripts were identified. Three of these appeared to encode immunity-related polypeptides, whereas 23 encoded hypothetical and/or new genes. Interestingly, different sets of transcripts appeared to be expressed in different subsets of hemocytes, as revealed by double-colored WISH. The 34 genes were categorized into two major subgroups based on their expression patterns during the C. intestinalis life cycle. Based on the gene expression profiles, we speculate that C. intestinalis hemocytes may exert more pleiotropic effects in immunity than previously believed.

Keywords: gene expression; ascidian; hemocytes; immunity; WISH; microarray.
Communicated by Satoshi Tabata
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