© 2001 by Kazusa DNA Research Institute
Complete Genomic Sequence of the Filamentous Nitrogen-fixing Cyanobacterium Anabaena sp. Strain PCC 7120
1Kazusa DNA Research Institute 1532-3 Yana, Kisarazu, Chiba 292-0812, Japan
2MSU-DOE Plant Research Laboratory, Michigan State University E. Lansing, MI 48824, USA
* To whom correspondence should be addressed. Tel. +81-438-52-3933, Fax. +81-438-52-3934
The nucleotide sequence of the entire genome of a filamentous cyanobacterium, Anabaena sp. strain PCC 7120, was determined. The genome of Anabaena consisted of a single chromosome (6,413,771 bp) and six plasmids, designated pCC7120
(408,101 bp), pCC7120ß (186,614 bp), pCC7120
(101,965 bp), pCC7120
(55,414 bp), pCC7120
(40,340 bp), and pCC7120
(5,584 bp). The chromosome bears 5368 potential protein-encoding genes, four sets of rRNA genes, 48 tRNA genes representing 42 tRNA species, and 4 genes for small structural RNAs. The predicted products of 45% of the potential protein-encoding genes showed sequence similarity to known and predicted proteins of known function, and 27% to translated products of hypothetical genes. The remaining 28% lacked significant similarity to genes for known and predicted proteins in the public DNA databases. More than 60 genes involved in various processes of heterocyst formation and nitrogen fixation were assigned to the chromosome based on their similarity to the reported genes. One hundred and ninety-five genes coding for components of two-component signal transduction systems, nearly 2.5 times as many as those in Synechocystis sp. PCC 6803, were identified on the chromosome. Only 37% of the Anabaena genes showed significant sequence similarity to those of Synechocystis, indicating a high degree of divergence of the gene information between the two cyanobacterial strains.
Key words: Anabaena sp. strain PCC 7120; genomic sequencing; heterocyst; nitrogen fixation
Current address: Life Sciences Division, Oak Ridge National Laboratory, MS-6480, Oak Ridge, TN 37830, USA
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  G. Shen, H. S. Leonard, W. M. Schluchter, and D. A. Bryant CpcM Posttranslationally Methylates Asparagine-71/72 of Phycobiliprotein Beta Subunits in Synechococcus sp. Strain PCC 7002 and Synechocystis sp. Strain PCC 6803 J. Bacteriol., July 15, 2008; 190(14): 4808 - 4817. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. D. Swingley, M. Chen, P. C. Cheung, A. L. Conrad, L. C. Dejesa, J. Hao, B. M. Honchak, L. E. Karbach, A. Kurdoglu, S. Lahiri, et al. Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina PNAS, February 12, 2008; 105(6): 2005 - 2010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kaneko, N. Nakajima, S. Okamoto, I. Suzuki, Y. Tanabe, M. Tamaoki, Y. Nakamura, F. Kasai, A. Watanabe, K. Kawashima, et al. Complete Genomic Structure of the Bloom-forming Toxic Cyanobacterium Microcystis aeruginosa NIES-843 DNA Res, January 11, 2008; (2008) dsm026v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Seki, M. Hanaoka, Y. Akimoto, S. Masuda, H. Iwasaki, and K. Tanaka Induction of a Group 2 {sigma} Factor, RPOD3, by High Light and the Underlying Mechanism in Synechococcus elongatus PCC 7942 J. Biol. Chem., December 21, 2007; 282(51): 36887 - 36894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Masukawa, K. Inoue, and H. Sakurai Effects of Disruption of Homocitrate Synthase Genes on Nostoc sp. Strain PCC 7120 Photobiological Hydrogen Production and Nitrogenase Appl. Envir. Microbiol., December 1, 2007; 73(23): 7562 - 7570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-H. Zhao, J. Zhang, J.-M. Tu, S. Bohm, M. Ploscher, L. Eichacker, C. Bubenzer, H. Scheer, X. Wang, and M. Zhou Lyase Activities of CpcS- and CpcT-like Proteins from Nostoc PCC7120 and Sequential Reconstitution of Binding Sites of Phycoerythrocyanin and Phycocyanin beta-Subunits J. Biol. Chem., November 23, 2007; 282(47): 34093 - 34103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Moslavac, K. Nicolaisen, O. Mirus, F. Al Dehni, R. Pernil, E. Flores, I. Maldener, and E. Schleiff A TolC-Like Protein Is Required for Heterocyst Development in Anabaena sp. Strain PCC 7120 J. Bacteriol., November 1, 2007; 189(21): 7887 - 7895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Awai, H. Watanabe, C. Benning, and I. Nishida Digalactosyldiacylglycerol is Required for Better Photosynthetic Growth of Synechocystis sp. PCC6803 Under Phosphate Limitation Plant Cell Physiol., November 1, 2007; 48(11): 1517 - 1523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-H. Zhao, P. Su, J.-M. Tu, X. Wang, H. Liu, M. Ploscher, L. Eichacker, B. Yang, M. Zhou, and H. Scheer Phycobilin:cystein-84 biliprotein lyase, a near-universal lyase for cysteine-84-binding sites in cyanobacterial phycobiliproteins PNAS, September 4, 2007; 104(36): 14300 - 14305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, S. Lechno-Yossef, Y. Gong, Q. Fan, C. P. Wolk, and X. Xu Predicted Glycosyl Transferase Genes Located outside the HEP Island Are Required for Formation of Heterocyst Envelope Polysaccharide in Anabaena sp. Strain PCC 7120 J. Bacteriol., July 15, 2007; 189(14): 5372 - 5378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Shi, J.-H. Li, Y. Cheng, L. Wang, W.-L. Chen, and C.-C. Zhang Two Genes Encoding Protein Kinases of the HstK Family Are Involved in Synthesis of the Minor Heterocyst-Specific Glycolipid in the Cyanobacterium Anabaena sp. Strain PCC 7120 J. Bacteriol., July 15, 2007; 189(14): 5075 - 5081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Valladares, I. Maldener, A. M. Muro-Pastor, E. Flores, and A. Herrero Heterocyst Development and Diazotrophic Metabolism in Terminal Respiratory Oxidase Mutants of the Cyanobacterium Anabaena sp. Strain PCC 7120 J. Bacteriol., June 15, 2007; 189(12): 4425 - 4430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zhao, Q. Guo, and J. Zhao A Membrane-Associated Mn-Superoxide Dismutase Protects the Photosynthetic Apparatus and Nitrogenase from Oxidative Damage in the Cyanobacterium Anabaena sp. PCC 7120 Plant Cell Physiol., April 1, 2007; 48(4): 563 - 572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yoshimura, S. Okamoto, Y. Tsumuraya, and M. Ohmori Group 3 sigma factor gene, sigJ, a key regulator of desiccation tolerance, regulates the synthesis of extracellular polysaccharide in cyanobacterium Anabaena sp. strain PCC 7120 DNA Res, March 21, 2007; (2007) dsm003v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakasugi, R. Alexova, C. J. Svenson, and B. A. Neilan Functional Analysis of PilT from the Toxic Cyanobacterium Microcystis aeruginosa PCC 7806 J. Bacteriol., March 1, 2007; 189(5): 1689 - 1697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. B. Panichkin, S. Arakawa-Kobayashi, T. Kanaseki, I. Suzuki, D. A. Los, S. V. Shestakov, and N. Murata Serine/Threonine Protein Kinase SpkA in Synechocystis sp. Strain PCC 6803 Is a Regulator of Expression of Three Putative pilA Operons, Formation of Thick Pili, and Cell Motility J. Bacteriol., November 1, 2006; 188(21): 7696 - 7699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lechno-Yossef, Q. Fan, S. Ehira, N. Sato, and C. P. Wolk Mutations in Four Regulatory Genes Have Interrelated Effects on Heterocyst Maturation in Anabaena sp. Strain PCC 7120 J. Bacteriol., November 1, 2006; 188(21): 7387 - 7395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Fan, S. Lechno-Yossef, S. Ehira, T. Kaneko, M. Ohmori, N. Sato, S. Tabata, and C. P. Wolk Signal Transduction Genes Required for Heterocyst Maturation in Anabaena sp. Strain PCC 7120. J. Bacteriol., September 1, 2006; 188(18): 6688 - 6693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sakr, M. Thyssen, M. Denis, and C.-C. Zhang Relationship among Several Key Cell Cycle Events in the Developmental Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol., August 1, 2006; 188(16): 5958 - 5965. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Wu, Z. Yang, and T. Kuang Impaired Photosynthesis in Phosphatidylglycerol-Deficient Mutant of Cyanobacterium Anabaena sp. PCC7120 with a Disrupted Gene Encoding a Putative Phosphatidylglycerophosphatase Plant Physiology, August 1, 2006; 141(4): 1274 - 1283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Awai, T. Kakimoto, C. Awai, T. Kaneko, Y. Nakamura, K.-i. Takamiya, H. Wada, and H. Ohta Comparative Genomic Analysis Revealed a Gene for Monoglucosyldiacylglycerol Synthase, an Enzyme for Photosynthetic Membrane Lipid Synthesis in Cyanobacteria Plant Physiology, July 1, 2006; 141(3): 1120 - 1127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Ashby and J. Houmard Cyanobacterial Two-Component Proteins: Structure, Diversity, Distribution, and Evolution Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 472 - 509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Makarova, E. V. Koonin, R. Haselkorn, and M. Y. Galperin Cyanobacterial response regulator PatA contains a conserved N-terminal domain (PATAN) with an alpha-helical insertion Bioinformatics, June 1, 2006; 22(11): 1297 - 1301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Johnson, W. Peti, T. Herrmann, I. A. Wilson, and K. Wuthrich Solution structure of Asl1650, an acyl carrier protein from Anabaena sp. PCC 7120 with a variant phosphopantetheinylation-site sequence Protein Sci., May 1, 2006; 15(5): 1030 - 1041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tomitani, A. H. Knoll, C. M. Cavanaugh, and T. Ohno The evolutionary diversification of cyanobacteria: Molecular-phylogenetic and paleontological perspectives PNAS, April 4, 2006; 103(14): 5442 - 5447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Gerdes, O. V. Kurnasov, K. Shatalin, B. Polanuyer, R. Sloutsky, V. Vonstein, R. Overbeek, and A. L. Osterman Comparative Genomics of NAD Biosynthesis in Cyanobacteria. J. Bacteriol., April 1, 2006; 188(8): 3012 - 3023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-H. Zhao, P. Su, J. Li, J.-M. Tu, M. Zhou, C. Bubenzer, and H. Scheer Chromophore Attachment to Phycobiliprotein beta-Subunits: PHYCOCYANOBILIN:CYSTEINE-beta84 PHYCOBILIPROTEIN LYASE ACTIVITY OF CpeS-LIKE PROTEIN FROM ANABAENA Sp. PCC7120 J. Biol. Chem., March 31, 2006; 281(13): 8573 - 8581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. S. Pratte and T. Thiel High-Affinity Vanadate Transport System in the Cyanobacterium Anabaena variabilis ATCC 29413 J. Bacteriol., January 15, 2006; 188(2): 464 - 468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Christiansen, R. Kurmayer, Q. Liu, and T. Borner Transposons Inactivate Biosynthesis of the Nonribosomal Peptide Microcystin in Naturally Occurring Planktothrix spp. Appl. Envir. Microbiol., January 1, 2006; 72(1): 117 - 123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Dvornyk Subfamilies of cpmA, a gene involved in circadian output, have different evolutionary histories in cyanobacteria Microbiology, January 1, 2006; 152(1): 75 - 84. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Murata and I. Suzuki Exploitation of genomic sequences in a systematic analysis to access how cyanobacteria sense environmental stress J. Exp. Bot., January 1, 2006; 57(2): 235 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Shi, T. S. Bibby, L. Jiang, A. J. Irwin, and P. G. Falkowski Protein Interactions Limit the Rate of Evolution of Photosynthetic Genes in Cyanobacteria Mol. Biol. Evol., November 1, 2005; 22(11): 2179 - 2189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ceballos-Chavez and A. Vioque Sequence-dependent Cleavage Site Selection by RNase Z from the Cyanobacterium Synechocystis sp. PCC 6803 J. Biol. Chem., September 30, 2005; 280(39): 33461 - 33469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jolley, A. Ben-Shem, N. Nelson, and P. Fromme Structure of Plant Photosystem I Revealed by Theoretical Modeling J. Biol. Chem., September 30, 2005; 280(39): 33627 - 33636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ballal and S. K. Apte Differential Expression of the Two kdp Operons in the Nitrogen-Fixing Cyanobacterium Anabaena sp. Strain L-31 Appl. Envir. Microbiol., September 1, 2005; 71(9): 5297 - 5303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. P. Dobrinski, D. L. Longo, and K. M. Scott The Carbon-Concentrating Mechanism of the Hydrothermal Vent Chemolithoautotroph Thiomicrospira crunogena J. Bacteriol., August 15, 2005; 187(16): 5761 - 5766. [Full Text] [PDF]
|
|
|
|
|
|
S. Ehira, M. Ohmori, and N. Sato Identification of Low-temperature-regulated ORFs in the Cyanobacterium Anabaena sp. Strain PCC 7120: Distinguishing the Effects of Low Temperature from the Effects of Photosystem II Excitation Pressure Plant Cell Physiol., August 1, 2005; 46(8): 1237 - 1245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ballal, M. Bramkamp, H. Rajaram, P. Zimmann, S. K. Apte, and K. Altendorf An Atypical KdpD Homologue from the Cyanobacterium Anabaena sp. Strain L-31: Cloning, In Vivo Expression, and Interaction with Escherichia coli KdpD-CTD J. Bacteriol., July 15, 2005; 187(14): 4921 - 4927. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Laurent, H. Chen, S. Bedu, F. Ziarelli, L. Peng, and C.-C. Zhang From the Cover: Nonmetabolizable analogue of 2-oxoglutarate elicits heterocyst differentiation under repressive conditions in Anabaena sp. PCC 7120 PNAS, July 12, 2005; 102(28): 9907 - 9912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zhang and L. Shi Distribution and evolution of multiple-step phosphorelay in prokaryotes: lateral domain recruitment involved in the formation of hybrid-type histidine kinases Microbiology, July 1, 2005; 151(7): 2159 - 2173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Suzuki, Y. Kanesaki, H. Hayashi, J. J. Hall, W. J. Simon, A. R. Slabas, and N. Murata The Histidine Kinase Hik34 Is Involved in Thermotolerance by Regulating the Expression of Heat Shock Genes in Synechocystis Plant Physiology, July 1, 2005; 138(3): 1409 - 1421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Tao, A. Schenzle, J. M. Odom, and Q. Cheng Novel Carotenoid Oxidase Involved in Biosynthesis of 4,4'-Diapolycopene Dialdehyde Appl. Envir. Microbiol., June 1, 2005; 71(6): 3294 - 3301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Burgdorf, E. van der Linden, M. Bernhard, Q. Y. Yin, J. W. Back, A. F. Hartog, A. O. Muijsers, C. G. de Koster, S. P. J. Albracht, and B. Friedrich The Soluble NAD+-Reducing [NiFe]-Hydrogenase from Ralstonia eutropha H16 Consists of Six Subunits and Can Be Specifically Activated by NADPH J. Bacteriol., May 1, 2005; 187(9): 3122 - 3132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Blanco-Rivero, F. Leganes, E. Fernandez-Valiente, P. Calle, and F. Fernandez-Pinas mrpA, a gene with roles in resistance to Na+ and adaptation to alkaline pH in the cyanobacterium Anabaena sp. PCC7120 Microbiology, May 1, 2005; 151(5): 1671 - 1682. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. J. Gehring New Perspectives on Eye Development and the Evolution of Eyes and Photoreceptors J. Hered., May 1, 2005; 96(3): 171 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ashida, Y. Sawa, and H. Shibata Cloning, Biochemical and Phylogenetic Characterizations of {gamma}-Glutamylcysteine Synthetase from Anabaena sp. PCC 7120 Plant Cell Physiol., April 1, 2005; 46(4): 557 - 562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Takaichi, M. Mochimaru, T. Maoka, and H. Katoh Myxol and 4-Ketomyxol 2'-Fucosides, not Rhamnosides, from Anabaena sp. PCC 7120 and Nostoc punctiforme PCC 73102, and Proposal for the Biosynthetic Pathway of Carotenoids Plant Cell Physiol., March 1, 2005; 46(3): 497 - 504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Kobayashi, S. Fujiwara, K. Shimogawara, C. Sakuma, Y. Shida, T. Kaise, H. Usuda, and M. Tsuzuki High Intracellular Phosphorus Contents Exhibit a Correlation with Arsenate Resistance in Chlamydomonas Mutants Plant Cell Physiol., March 1, 2005; 46(3): 489 - 496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Huang, Q. Fan, S. Lechno-Yossef, E. Wojciuch, C. P. Wolk, T. Kaneko, and S. Tabata Clustered Genes Required for the Synthesis of Heterocyst Envelope Polysaccharide in Anabaena sp. Strain PCC 7120 J. Bacteriol., February 1, 2005; 187(3): 1114 - 1123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Oldham, A. R. Brash, and M. E. Newcomer The structure of coral allene oxide synthase reveals a catalase adapted for metabolism of a fatty acid hydroperoxide PNAS, January 11, 2005; 102(2): 297 - 302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Iwai, H. Katoh, M. Katayama, and M. Ikeuchi PSII-Tc Protein Plays an Important Role in Dimerization of Photosystem II Plant Cell Physiol., December 15, 2004; 45(12): 1809 - 1816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Rondon, K. S. Ballering, and M. G. Thomas Identification and analysis of a siderophore biosynthetic gene cluster from Agrobacterium tumefaciens C58 Microbiology, November 1, 2004; 150(11): 3857 - 3866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Roose and H. B. Pakrasi Evidence that D1 Processing Is Required for Manganese Binding and Extrinsic Protein Assembly into Photosystem II J. Biol. Chem., October 29, 2004; 279(44): 45417 - 45422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Y. Galperin and E. V. Koonin 'Conserved hypothetical' proteins: prioritization of targets for experimental study Nucleic Acids Res., October 12, 2004; 32(18): 5452 - 5463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Chin, V. Valinluck, S. Magaki, and J. Ryu KpnBI is the prototype of a new family (IE) of bacterial type I restriction-modification system Nucleic Acids Res., October 8, 2004; 32(18): e138 - e138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wu, D. Liu, M. H. Lee, and J. W. Golden patS Minigenes Inhibit Heterocyst Development of Anabaena sp. Strain PCC 7120 J. Bacteriol., October 1, 2004; 186(19): 6422 - 6429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Stavrinides and D. S. Guttman Nucleotide Sequence and Evolution of the Five-Plasmid Complement of the Phytopathogen Pseudomonas syringae pv. maculicola ES4326 J. Bacteriol., August 1, 2004; 186(15): 5101 - 5115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Baier, H. Lehmann, D. P. Stephan, and W. Lockau NblA is essential for phycobi |