Skip Navigation

DNA Research 2001 8(1):23-31; doi:10.1093/dnares/8.1.23
© 2001 by Kazusa DNA Research Institute
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Fukasawa, T.
Right arrow Articles by Sakurai, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fukasawa, T.
Right arrow Articles by Sakurai, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

A Genome-wide Analysis of Transcriptional Effect of Gal11 in Saccharomyces cerevisiae: An Application of "Mini-array Hybridization Technique"

Toshio Fukasawa1,*, Mariko Fukuma2, Ken-ichi Yano3 {dagger} and Hiroshi Sakurai4

1Department of Microbiology Japan
2Department of Pathology, Keio University School of Medicine Shinjuku,Tokyo 160-8582, Japan
3Department of Microbiology,Kazusa DNA Research Institute, Kisarazu Chiba 292-0812, Japan
4Department of Microbiology,,School of Health Sciences, Faculty of Medicine, Kanazawa University Kanazawa, Ishikawa 920-0943, Japan

* To whom correspondence should be addressed. 2-8-2 Maeharanishi,Funabashi, Chiba 274-0825 Japan. Tel. & Fax. +81-47-477-6948, E-mail: toshfuka{at}interlink.or.jp

The Gal11 protein is a subunit of the Mediator complex. Biochemical as well as genetic studies have strongly suggested that Gal11 is a positive global regulator of transcription. Some reports argue that Gal11 is a negative regulator, however. Here we have adopted the "Mini-array membrane hybridization" to analyze the effect of Gal11 in a genome-wide fashion. This technique has been demonstrated to be reliable to identify genes whose expression is controlled by a specific set of genetic and/or physiological signals. Our experiments indicate that this technique is applicable to profile the gene expression in yeast grown in rich medium. Thus mRNAs of 40% of significantly expressed genes are reduced more than two fold in gal11null yeast, in which only 3% of mRNAs are increased more than two fold. These results strongly suggest that Gal11 functions globally as a positive regulator in vivo.

Key words: genome-wide analysis; Mddiator; Gal11; Rpbl; DNA-array

{dagger} Present address: Institute of Japanese Foundation for Cancer Research, Tokyo 170-8455, Japan


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
D.-H. Kim, G. S. Kim, C. H. Yun, and Y. C. Lee
Functional Conservation of the Glutamine-Rich Domains of Yeast Gal11 and Human SRC-1 in the Transactivation of Glucocorticoid Receptor Tau 1 in Saccharomyces cerevisiae
Mol. Cell. Biol., February 1, 2008; 28(3): 913 - 925.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Yamamoto, Y. Mizukami, and H. Sakurai
Identification of a Novel Class of Target Genes and a Novel Type of Binding Sequence of Heat Shock Transcription Factor in Saccharomyces cerevisiae
J. Biol. Chem., March 25, 2005; 280(12): 11911 - 11919.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Matsumoto, T. Obayashi, Y. Sasaki-Sekimoto, H. Ohta, K.-i. Takamiya, and T. Masuda
Gene Expression Profiling of the Tetrapyrrole Metabolic Pathway in Arabidopsis with a Mini-Array System
Plant Physiology, August 1, 2004; 135(4): 2379 - 2391.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Badi and A. Barberis
The CUP1 upstream repeated element renders CUP1 promoter activation insensitive to mutations in the RNA polymerase II transcription complex
Nucleic Acids Res., March 15, 2002; 30(6): 1306 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
L. T. Bhoite, Y. Yu, and D. J. Stillman
The Swi5 activator recruits the Mediator complex to the HO promoter without RNA polymerase II
Genes & Dev., September 15, 2001; 15(18): 2457 - 2469.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.