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DNA Research 2000 7(3):157-163; doi:10.1093/dnares/7.3.157
© 2000 by Kazusa DNA Research Institute
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Analysis of Unique Variable Region of a Plant Root Inducing Plasmid, pRi1724, by the Construction of its Physical Map and Library

Kazuki Moriguchi1, Yasunobu Maeda1, Megumi Satou1, Miho Kataoka1, Nobukazu Tanaka2 and Kazuo Yoshida1,*

1Department of Biological Science, Graduate School of Science, Hiroshima University 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8526, Japan
2Center for Gene Science, Hiroshima University 1-4-2 Kagamiyama, Higashi-Hiroshima 739-8527, Japan

* To whom correspondence should be addressed. Tel. +81-824-24-7455, Fax. +81-824-24-0734, E-mail: kyoshida{at}sci.hiroshima-u.ac.jp

Ri plasmids in Agrobacterium rhizogenes specifically induce the hairy root syndrome on various dicotyledonous plants. Its T-DNA transfer system as well as those of Ti plasmids have successfully provided the fundamental technique to introduce exogenous genes into plants. To study the Ri genome structure, we constructed a complete BamHI physical map and a lambda library of pRi1724 of A. rhizogenes strain 1724. By using these, we carried out the complete sequence of the 74-kb region between the right border of TDNA and tra operon, which is the highly variable region (VAR) among Ri and Ti plasmids. As a result, we found three kinds of putative ABC-type transport operons, histidine utilization operon, glycerol utilization operon and two chemoreceptor genes. In addition, a virulence-related gene, tzs was located independently of the vir region.

Key words: Ri plasmid; Agrobacterium rhizogenes; Physical map; VAR (variable region)


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