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DNA Research 2000 7(2):127-130; doi:10.1093/dnares/7.2.127
© 2000 by Kazusa DNA Research Institute
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Generation of 7137 Non-redundant Expressed Sequence Tags from a Legume, Lotus japonicus

Erika Asamizu, Yasukazu Nakamura, Shusei Sato and Satoshi Tabata*

Kazusa DNA Research Institute 1532-3 Yana, Kisarazu, Chiba 292-0812, Japan

For comprehensive analysis of genes expressed in a model legume, Lotus japonicus, a total of 22,983 5' end expressed sequence tags (ESTs) were accumulated from normalized and size-selected cDNA libraries constructed from young (2 weeks old) plants. The EST sequences were clustered into 7137 non-redundant groups. Similarity search against public non-redundant protein database indicated that 3302 groups showed similarity to genes of known function, 1143 groups to hypothetical genes, and 2692 were novel sequences. Homologues of 5 nodule-specific genes which have been reported in other legume species were contained in the collected ESTs, suggesting that the EST source generated in this study will become a useful tool for identification of genes related to legume-specific biological processes. The sequence data of individual ESTs are available at the web site: http://www.kazusa.or.jp/en/plant/lotus/EST/.

Key words: Legume; Lotus japonicus; cDNA; EST

* To whom correspondence should be addressed. Tel. +81-438-52-3933, Fax. +81-438-52-3934, E-mail: tabata{at}kazusa.or.jp


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