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DNA Research 1999 6(2):103-108; doi:10.1093/dnares/6.2.103
© 1999 by Kazusa DNA Research Institute
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The Gene for an Exopolyphosphatase of Pseudomonas aeruginosa

Tatsushi Miyake1, Toshikazu Shiba1,*, Atsushi Kameda1, Yoshiharu Ihara1, Masanobu Munekata1, Kazuya Ishige2 and Toshitada Noguchi2

1Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University Sapporo 060-8628, Japan
2Biochemicals Division, YAMASA Corporation Choshi, Chiba 288-0056, Japan

* To whom correspondence should be addressed. Tel. & Fax. +81-11-706-7816, E-mail: shiba{at}dove-mc.eng.hokudai.ac.jp

In Pseudomonas aeriginosa, a gene, ppx, that encodes exopolyphosphatase [exopoly(P)ase; EC 3.6.1.11] of 506 amino acids (56,419 Da) was found downstream of the gene for polyphosphate kinase, ppk. Since ppx is located in the opposite direction of the ppk gene, they do not constitute an operon. The predicted amino acid sequence of PPX is 41% identical with Escherichia coli PPX. The gene product of ppx(paPPX) was overproduced in E. coli, and its activity was evaluated. Orthophosphate (Pi) is released from polyphosphate [poly(P)], the average chain lengths of which are 79 and 750, respectively. The amount of Pi released matched the amount of poly(P) lost. Thus ppx encodes an enzyme that has exopoly(P)ase activity.

Key words: Exopolyphosphatase; inorganic polyphosphate; Pseudomonas aeruginosaPAO1


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