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DNA Research 1998 5(3):163-168; doi:10.1093/dnares/5.3.163
© 1998 by Kazusa DNA Research Institute
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A Physical Map of Arabidopsis thaliana Chromosome 3 Represented by Two Contigs of CIC YAC, P1, TAC and BAC Clones

Shusei Sato1, Hirokazu Kotani1, Reiko Hayashi1,2, Yao-Guang Liu3,4, Daisuke Shibata3 and Satoshi Tabata1,*

1Kazusa DNA Research Institute 1532-3 Yana, Kisarazu, Chiba 292-0812, Japan
2Chiba Prefectural Agricultural Experiment Station 2-325 Sawara, Sawara, Chiba 287-0822, Japan
3Mitusi Plant Biotechnology Research Institute TCI D-21, 2-1-6 Sengen, Tsukuba, Ibaraki 305-0047, Japan
4Genetic Engineering Laboratory, South China Agricultural University Guangzhou 510642, People's Republic of China

* To whom correspondence should be addressed. Tel. +81-438-52-3933, Fax. +81-438-52-3934, E-mail: tabata{at}kazusa.or.jp

We have constructed a physical map of Arabidopsis thaliana chromosome 3 by ordering the clones from CIC YAC, P1, TAC and BAC libraries using the sequences of a variety of genetic and EST markers and terminal sequences of clones. The markers used were 112 DNA markers, 145 YAC end sequences, and 156 end sequences of P1, TAC and BAC clones. The entire genome of chromosome 3, except for the centromeric and telomeric regions, was covered by two large contigs, 13.6 Mb and 9.2 Mb long. This physical map will facilitate map-based cloning experiments as well as genome sequencing of chromosome 3. The map and end sequence information are available on the KAOS (Kazusa Arabidopsis data Opening Site) web site at http://www.kazusa.or.jp/arabi/.

Key words: Arabidopsis thaliana; chromosome 3; physical map; contig map


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