Skip Navigation

DNA Research 1998 5(2):77-85; doi:10.1093/dnares/5.2.77
© 1998 by Kazusa DNA Research Institute
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Haidaris, C. G.
Right arrow Articles by Simpson-Haidaris, P. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haidaris, C. G.
Right arrow Articles by Simpson-Haidaris, P. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Characterization of Mouse Pneumocystis carinii Surface Glycoprotein A

Constantine G. Haidaris1,*, Oliver F. Medzihradsky1, Francis Gigliotti1,3 and Patricia J. Simpson-Haidaris1,2,4

1Departments of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry Box 672, 601 Elmwood Ave., Rochester, NY 14642, USA
2Medicine/Vascular Medicine Unit, University of Rochester School of Medicine and Dentistry Box 672, 601 Elmwood Ave., Rochester, NY 14642, USA
3Department of Pediatrics, University of Rochester School of Medicine and Dentistry Box 672, 601 Elmwood Ave., Rochester, NY 14642, USA
4Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry Box 672, 601 Elmwood Ave., Rochester, NY 14642, USA

* To whom correspondence should be addressed. Tel. +1-716-275-0678, Fax. +1-716-473-9573, E-mail: haid{at}uhura.cc.rochester.edu

Since the mouse offers an easily manipulated experimental animal model for the study of the immunopathogenesis of pneumonia caused by the opportunist Pneumocystis carinii, we cloned and characterized cDNAs encoding an abundant, immunogenic surface antigen termed glycoprotein A (gpA) from mouse P. carinii. A cDNA library was constructed in bacteriophage {lambda}gt11 from P. carinii-infected mouse lung poly(A+) RNA. Using a nucleic acid probe derived from a conserved region of the mouse P. carinii gpA structural gene, cDNAs encoding gpA were identified. A composite full-length gpA coding sequence was assembled from two overlapping cDNA clones. A DNA element homologous to the rat P. carinii upstream conserved sequence (UCS) was identified at the 5' end of several of the mouse P. carinii gpA cDNA clones, just upstream of the sequences encoding gpA structural gene isoforms. Using primer extension analysis, two neighboring putative transcriptional start sites were located on UCS-gpA mRNAs approximately 25 and 30 nt, respectively, upstream of the most 5' gpA cDNA clone isolated, suggesting a 5' UCS of 489 or 494 nucleotides in mouse P. carinii gpA. A comparative alignment of the composite mouse P. carinii gpA deduced amino acid sequence with gpA homologs from rat, human and ferret P. carinii demonstrated 156 identical residues, including 46 cysteines, further supporting the hypothesis for conserved secondary structure, as well as function, for gpA from all P. carinii.

Key words: Pneumocystis; glycoprotein A; major surface glycoprotein; cDNA cloning; mouse


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
J. Wells, F. Gigliotti, P. J. Simpson-Haidaris, and C. G. Haidaris
Epitope Mapping of a Protective Monoclonal Antibody against Pneumocystis carinii with Shared Reactivity to Streptococcus pneumoniae Surface Antigen PspA
Infect. Immun., March 1, 2004; 72(3): 1548 - 1556.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
J. K. Schaffzin and J. R. Stringer
Expression of the Pneumocystis carinii major surface glycoprotein epitope is correlated with linkage of the cognate gene to the upstream conserved sequence locus
Microbiology, March 1, 2004; 150(3): 677 - 686.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. R. Stringer and S. P. Keely
Genetics of Surface Antigen Expression in Pneumocystis carinii
Infect. Immun., February 1, 2001; 69(2): 627 - 639.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Guadiz, C. G. Haidaris, G. N. Maine, and P. J. Simpson-Haidaris
The Carboxyl Terminus of Pneumocystis carinii Glycoprotein A Encodes a Functional Glycosylphosphatidylinositol Signal Sequence
J. Biol. Chem., October 2, 1998; 273(40): 26202 - 26209.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
Q. Mei, R. E. Turner, V. Sorial, D. Klivington, C. W. Angus, and J. A. Kovacs
Characterization of Major Surface Glycoprotein Genes of Human Pneumocystis carinii and High-Level Expression of a Conserved Region
Infect. Immun., September 1, 1998; 66(9): 4268 - 4273.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.