© 1998 by Kazusa DNA Research Institute
A Novel Superoxide Dismutase Gene Encoding Membrane-bound and Extracellular Isoforms by Alternative Splicing in Caenorhabditis elegans
1Department of Biochemistry, Kihara Institute for Biological Research, Yokohama City University Totsukaku, Maioka-cho, Yokohama 244, Japan
2Department of Molecular Life Science, Tokai University School of Medicine Isehara, Kanagawa 259-11, Japan
* To whom correspondence should be addressed. Tel. +81-45-820-1906, Fax. +81-45-820-1901, E-mail: dayusawa{at}yokohama-cu.ac.jp
We have identified a novel Cu/Zn superoxide dismutase gene (termed SOD-4) in Caenorhabditis elegans. Characterization of its complementary DNA revealed that the gene encodes two isoforms by alternative splicing, SOD4-1 and SOD4-2 which differ in their C-terminal exons. Their predicted amino acid sequences include a consensus signal peptide at their N-termini and are homologous to the extracellular-types of Cu/Zn superoxide dismutase in mammals. In addition, SOD4-2 possesses a putative transmembrane domain at the C-terminal region. When transiently expressed in Chinese hamster ovary cells, both types were found in the membranes and SOD4-1 also in the culture fluid. It is, therefore, indicated that SOD4-1 is an extracellular form and SOD4-2 a membrane-bound form, the latter representing a novel type of SOD. In C. elegans, SOD4-2 mRNA was found to be preferentially expressed in eggs.
Key words: aging; mev-1 mutant; gene transfer; development; superoxide
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