© 1996 by Kazusa DNA Research Institute
A 460-kb DNA Sequence of the Escherichia coli K-12 Genome Corresponding to the 40.1–50.0 min Region on the Linkage Map
1Research and Education Center for Genetic Information, Nara Institute of Science and Technology 8916-5 Takayama-cho, Ikoma 630-01, Japan
2Department of Molecular Biology, School of Science, Nagoya University Furo-cho, Chikusaku, Nagoya 464-01, Japan
3Department of Biology, Faculty of Science, Kobe University 1-1 Rokkodai-cho, Nadaku, Kobe 657, Japan
4National Institute for Basic Biology 38 Nishigonaka, Myodaiji-cho, Okazaki 444, Japan
5Research Institute for Microbial Diseases, Osaka University 3-1 Yamadagaoka, Suita 565, Japan
6Faculty of Pharmaceutical Sciences, Kyushu University 3-1-1 Maedashi, Higashiku, Fukuoka 812-82, Japan
7Department of Applied Physics and Chemistry, University of Electro-Communications 1-5-1 Chofugaoka, Chofu 182, Japan
8Institute of Medical Science, University of Tokyo 4-6-1 Shiroganedai, Minatoku, Tokyo 108, Japan
9Department of BioSciences, Teikyo University 1-1 Toyosatodai, Utsunomiya 320, Japan
10Institute for Virus Research, Kyoto University Kawahara-cho, Shogoin, Sakyoku, Kyoto 606-01, Japan
11Department of Genetics, Hyogo College of Medicine 1-1 Mukogawa-cho, Nishinomiya 663, Japan
* To whom correspondence should be addressed. Tel. and Fax. +81-564-55-7690, E-mail; kishori{at}nibb.ac.jp
The 465,813 base pair sequence corresponding to the 40.1–50.0 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. Analysis of the sequence revealed that this region contained at least 466 potential open reading frames, of which 187 (40%) were previously reported, 105 (23%) were homologous to other known genes, 103 (22%) were identical or similar to hypothetical genes registered in databases, and the remaining 71 (15%) did not show a significant similarity to any other gene. At the 45.2–46.0 min region, we found a very large cluster of about 30 genes, whose functions are involved in the biosynthesis of polysaccharides as the components of outer membranes. In addition, we identified anew asn-tRNA gene, designated asnW, between the asnT and asnU genes and a new lysogenic phage attachment site as the cis-element.
Key words: Escherichia coli K12; genome sequence; ORF analysis
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