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DNA Research Advance Access originally published online on February 22, 2006
DNA Research 2006 13(1):25-35; doi:10.1093/dnares/dsi025
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© The Author 2006. Kazusa DNA Research Institute
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Identification of Thirty-four Transcripts Expressed Specifically in Hemocytes of Ciona intestinalis and Their Expression Profiles throughout the Life Cycle

Michio Ogasawara1, Noriko Nakazawa1, Kaoru Azumi2, Eiji Yamabe3, Nori Satoh4 and Masanobu Satake3,*

1 Department of Biology, Faculty of Science, Chiba University 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan
2 Division of Innovative Research, Creative Research Initiative "Sousei’, Hokkaido University Sapporo 001-0021, Japan
3 Institute of Development, Aging and Cancer, Tohoku University Seiryo-machi 4-1, Aoba-ku, Sendai 980-8575, Japan
4 Department of Zoology, Graduate School of Science, Kyoto University Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan

The innate immunity of ascidian hemocytes is considered to be a prototype of that in vertebrates. In this study, we identified as many transcripts as possible that were expressed specifically in hemocytes of Ciona intestinalis, a ubiquitous species of ascidian. Using a large-scale whole-mount in situ hybridization (WISH) technique and young adult specimens of C. intestinalis, 34 such transcripts were identified. Three of these appeared to encode immunity-related polypeptides, whereas 23 encoded hypothetical and/or new genes. Interestingly, different sets of transcripts appeared to be expressed in different subsets of hemocytes, as revealed by double-colored WISH. The 34 genes were categorized into two major subgroups based on their expression patterns during the C. intestinalis life cycle. Based on the gene expression profiles, we speculate that C. intestinalis hemocytes may exert more pleiotropic effects in immunity than previously believed.

Key words: gene expression; ascidian; hemocytes; immunity; WISH; microarray

*To whom correspondence should be addressed. Tel. +81-22-717-8477, Fax. +81-22-717-8482, E-mail: satake{at}idac.tohoku.ac.jp

Communicated by Satoshi Tabata


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