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DNA Research Advance Access published online on August 27, 2008
DNA Research, doi:10.1093/dnares/dsn019
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© The Author 2008. Kazusa DNA Research Institute
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Superpositioning of Deletions Promotes Growth of Escherichia coli with a Reduced Genome

Hiroshi Mizoguchi1,*, Yoshie Sawano1, Jun-ichi Kato2 and Hideo Mori1,*

1 Biofrontier Laboratories, Kyowa Hakko Kogyo Co. Ltd, 3-6-6 Asahimachi, Machidashi, Tokyo 194-8533, Japan
2 Department of Biological Sciences, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan

Received 18 December 2007 ; accepted 9 July 2008.

Escherichia coli has dispensable genome regions and eliminating them may improve cell use by reducing unnecessary metabolic pathways and complex regulatory networks. Although several strains with reduced genomes have already been constructed, there have been no reports of strains constructed with deletions assayed for influence on growth. To retain robust growth and fundamental metabolic pathways, the growth of each deletion strain and combination effects of deletions were checked using M9 minimal medium. Then a new strain, MGF-01, with a 1 Mb reduced genome was constructed by integrating deletions that did not affect growth. MGF-01 grew as well as the wild type in the exponential phase and continued growing after the wild type had entered the stationary phase. The final cell density of MGF-01 was 1.5 times higher than that of the wild-type strain. Using MGF-01 as a production host, a 2.4-fold increase in L-threonine production was achieved.

* To whom correspondence should be addressed. Tel. +81 42-725-2555. Fax. +81 42-726-8330. E-mail: mizoguchi.hiroshi{at}kyowa.co.jp (hiroshi.mizoguchi{at}kyowa-kirin.co.jp), hmori{at}kyowa.co.jp (hideo.mori{at}kyowa-kirin.co.jp)

Edited by Naotake Ogasawara


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