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DNA Research Advance Access published online on May 29, 2008
DNA Research, doi:10.1093/dnares/dsn012
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© The Author 2008. Kazusa DNA Research Institute
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Soybean Seed Extracts Preferentially Express Genomic Loci of Bradyrhizobium japonicum in the Initial Interaction with Soybean, Glycine max (L.) Merr

Min Wei1, Tadashi Yokoyama2, Kiwamu Minamisawa3, Hisayuki Mitsui3, Manabu Itakura3, Takakazu Kaneko4, Satoshi Tabata4, Kazuhiko Saeki5, Hirofumi Omori6, Shigeyuki Tajima7, Toshiki Uchiumi8, Mikiko Abe8 and Takuji Ohwada1,*

1 Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
2 Tokyo University of Agriculture and Technology, Saiwai-cho, Fuchu 183-8509, Tokyo, Japan
3 Graduate School of Life Science, Tohoku University, Aoba-ku, Sendai 980-8577, Japan
4 Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Chiba 292-0812, Japan
5 Department of Biological Science, Faculty of Science, Nara Women's University, Kitauoyanishi-machi, Nara 630-8506, Japan
6 Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan
7 Department of Life Science, Kagawa University, Miki-cho, Kagawa 761-0795, Japan
8 Department of Chemistry and Bioscience, Faculty of Science, Kagoshima University, Kagoshima 890-0065, Japan

Received 27 December 2007 ; accepted 2 May 2008.

Initial interaction between rhizobia and legumes actually starts via encounters of both partners in the rhizosphere. In this study, the global expression profiles of Bradyrhizobium japonicum USDA 110 in response to soybean (Glycine max) seed extracts (SSE) and genistein, a major soybean-released isoflavone for nod genes induction of B. japonicum, were compared. SSE induced many genomic loci as compared with genistein (5.0 µM), nevertheless SSE-supplemented medium contained 4.7 µM genistein. SSE markedly induced four predominant genomic regions within a large symbiosis island (681 kb), which include tts genes (type III secretion system) and various nod genes. In addition, SSE-treated cells expressed many genomic loci containing genes for polygalacturonase (cell-wall degradation), exopolysaccharide synthesis, 1-aminocyclopropane-1-carboxylate deaminase, ribosome proteins family and energy metabolism even outside symbiosis island. On the other hand, genistein-treated cells exclusively showed one expression cluster including common nod gene operon within symbiosis island and six expression loci including multidrug resistance, which were shared with SSE-treated cells. Twelve putatively regulated genes were indeed validated by quantitative RT-PCR. Several SSE-induced genomic loci likely participate in the initial interaction with legumes. Thus, these results can provide a basic knowledge for screening novel genes relevant to the B. japonicum- soybean symbiosis.

Key words: soybean seed extracts; Bradyrhizobium japonicum; expression clusters; genistein; symbiosis

* To whom correspondence should be addressed. Tel. +81 155-49-5552. Fax. +81 155-49-5577. E-mail: taku{at}obihiro.ac.jp

Edited by Masahira Hattori


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