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DNA Research Advance Access published online on January 7, 2008
DNA Research, doi:10.1093/dnares/dsm024
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© The Author 2008. Kazusa DNA Research Institute
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

A Genome-wide Approach to Identify the Genes Involved in Biofilm Formation in E. coli

Emma Tabe Eko Niba1, Yoshiaki Naka1, Megumi Nagase2, Hirotada Mori3 and Madoka Kitakawa2,*

1 Graduate School of Science and Technology, Kobe University, Kobe, Hyogo 657-8501, Japan
2 Faculty of Science, Department of Biology, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, Hyogo 657-8501, Japan
3 Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan

Received 17 October 2007 ; accepted 25 November 2007.

Biofilm forming cells are distinctive from the well-investigated planktonic cells and exhibit a different type of gene expression. Several new Escherichia coli genes related to biofilm formation have recently been identified through genomic approaches such as DNA microarray analysis. However, many others involved in this process might have escaped detection due to poor expression, regulatory mechanism, or genetic backgrounds. Here, we screened a collection of single-gene deletion mutants of E. coli named ‘Keio collection’ to identify genes required for biofilm formation. Of the 3985 mutants of non-essential genes in the collection thus examined, 110 showed a reduction in biofilm formation nine of which have not been well characterized yet. Systematic and quantitative analysis revealed the involvement of genes of various functions and reinforced the importance in biofilm formation of the genes for cell surface structures and cell membrane. Characterization of the nine mutants of function-unknown genes indicated that some of them, such as yfgA that genetically interacts with a periplasmic chaperone gene surA together with yciB and yciM, might be required for the integrity of outer membrane.

Key words: biofilm; deletion mutant; E. coli

* To whom correspondence should be addressed. Tel. +81 78-803-5724. Fax. +81 78-803-5783. E-mail: madoka{at}kobe-u.ac.jp

Edited by Dr. Naotake Ogasawara


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