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DNA Research 2000 7(6):357-366; doi:10.1093/dnares/7.6.357
© 2000 by Kazusa DNA Research Institute
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Characterization of Long cDNA Clones from Human Adult Spleen

Atsushi Hattori2, Koji Okumura2, Takahiro Nagase1, Reiko Kikuno, Makoto Hirosawa1 and Osamu Ohara1,*

1Kazusa DNA Research Institute 1532-3 Yana, Kisarazu, Chiba 292-0812, Japan
2Aisin Cosmos R & D Co. Ltd. 1698 Yana, Kisarazu, Chiba 292-0812, Japan

* To whom correspondence should be addressed. Tel. +81-438-52-3913, Fax. +81-438-52-3914, E-mail: ohara{at}kazusa.or.jp

As an extension of our analysis of long cDNAs, we here report the characterization of cDNA clones from human adult spleen. From 2000 cDNA clones randomly sampled from a size-fractionated human spleen cDNA library (average size 4.5 kb), 97 clones were selected for sequencing according to their ability to code for protein at the 5'-endsequences and the novelty of their endsequences. The sequence data of these clones demonstrated that 87 out of 97 cDNA clones were derived from independent human genes. The average sizes of the inserts and corresponding open reading frames of these 87 cDNAs reached 4.5 kb and 1.4 kb (corresponding to 468 amino acid residues), respectively. In addition to these sequence analyses in silico, the expression profiles of the genes were also studied in ten human adult tissues by reverse transcriptioncoupledp olymerase chain reaction, products of which were quantified by enzyme-linkedimm unosorbent assay. The results indicated that spleen could be used as an additional source of human long cDNAs to complement the list of human genes.

Key words: long cDNA; single-pass sequence; cDNA sequencing; expression profile; alternative splicing; spleen


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