© 2000 by Kazusa DNA Research Institute
Prediction of the Coding Sequences of Unidentified Human Genes. XIX. The complete Sequences of 100 New cDNA Clones from Brain Which Code for Large Proteins in vitro
1Kazusa DNA Research Institute 1532-3 Yana, Kisarazu, Chiba 292-0812, Japan
2Aisin Cosmos R & D, Co. Ltd. 1698 Yana, Kisarazu, Chiba 292-0812, Japan
* To whom correspondence should be addressed. Tel. +81-438-52-3913, Fax. +81-438-52-3914, E-mail: ohara{at}kazusa.or.jp
As an extension of our human cDNA project for accumulating sequence information on the coding sequences of unidentified genes, we here present the entire sequences of 100 cDNA clones of unidentified genes, named KIAA1673-KIAA1772, from three sets of size-fractionated cDNA libraries derived from human adult whole brain, hippocampus, and fetal whole brain. The average sizes of the inserts and corresponding open reading frames of cDNA clones analyzed here were 4.9 kb and 2.7 kb (corresponding to 895 amino acid residues), respectively. By computer-assisted analysis of the deduced amino acid sequences, 44 predicted gene products were classified into five functional categories of proteins relating to cell signaling/communication, nucleic acid management, cell structure/motility, protein management, and metabolism. Furthermore, the expression profiles of the genes were also studied in 10 human tissues, 8 brain regions, spinal cord, fetal brain and fetal liver by reverse-transcription-coupled polymerase chain reaction, the products of which were quantified by enzyme-linked immunosorbent assay.
Key words: large proteins; in vitro transcription/translation; cDNA sequencing; expression profile; chromosomal location; brain
Present address: Advanced Engineering Center, RIKEN (The Institute of Physical and Chemical Research), 2-1, Hirosawa, Wako, Saitama 351-0918, Japan
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