Skip Navigation

DNA Research 2000 7(6):339-345; doi:10.1093/dnares/7.6.339
© 2000 by Kazusa DNA Research Institute
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Gispert, S.
Right arrow Articles by Nussbaum, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gispert, S.
Right arrow Articles by Nussbaum, R. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Cloning and Genomic Organization of the Mouse Gene Slc23a1 Encoding a Vitamin C Transporter

Suzana Gispert1, Amalia Dutra1, Andrew Lieberman2, Dan Friedlich3 and Robert L. Nussbaum1,*

1Genetic Disease Research Branch, National Human Genome Research Institute
2Neurogenetics Branch, National Institute of Neurological Disease and Stroke
3Howard Hughes Medical Institute, NIH Bethesda, Maryland 20892, USA

* To whom correspondence should be addressed. Genetic Disease Research Branch, Building 49, Room 4A72 NIH, 49 Convent Drive, Bethesda, MD 20892-4472, USA. Tel. +1-301-402-2039, Fax. +1-301-402-2170, E-mail: rlnuss{at}nhgri.nih.gov

Vitamin C is known to exist in particularly high concentrations in brain tissue, and its free radical scavenging function is thought to represent a major antioxidative defense system. We have cloned, sequenced and analyzed the genomic structure of a mouse sodium-dependent vitamin Ctransp orter gene, Slc23a1 (also known as Svct2). The mouse Slc23a1 cDNA is 6.4 kb long and was cloned directly from a mouse brain RNA preparation. Hybridization screening of a mouse genomic BAClibrary identified BAC 53L21 whichcontains at least the entire coding sequence of the mouse Slc23a1 gene. Determination of the exon-intron structure of the gene revealed 17 exons ranging from 58 bp to 4407 bp extending over 50 kb of the mouse genome, with the translation start codon located in exon 3. Its 1944 nucleotide open reading frame encodes a polypeptide of 647 aa, which is highly similar to rat and human orthologs. The mouse gene was assigned to chromosome 2qG2 by fluorescence in situ hybridization analysis. Expression of this gene was demonstrated in a wide range of tissues, with especially high levels in brain. Neurodegenerative diseases with an established role for oxidative stress in the cytoplasm may therefore be conditions of SLC23A1 dysfunction.

Key words: gene structure; Vitamin C; transporter; oxidative stress


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Nutr.Home page
S.-M. Kuo, M. E. MacLean, K. McCormick, and J. X. Wilson
Gender and Sodium-Ascorbate Transporter Isoforms Determine Ascorbate Concentrations in Mice
J. Nutr., September 1, 2004; 134(9): 2216 - 2221.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. L. Crowe, D. Rana, F. Fraser, I. Bancroft, and M. Trick
BACFinder: genomic localisation of large insert genomic clones based on restriction fingerprinting
Nucleic Acids Res., November 1, 2002; 30(21): e118 - e118.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.