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DNA Research 2000 7(6):323-330; doi:10.1093/dnares/7.6.323
© 2000 by Kazusa DNA Research Institute
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Complete Structure of the Chloroplast Genome of a Legume, Lotus japonicus

Tomohiko Kato, Takakazu Kaneko, Shusei Sato, Yasukazu Nakamura and Satoshi Tabata*

Kazusa DNA Research Institute 1532-3 Yana, Kisarazu, Chiba 292-0812, Japan

* To whom correspondence should be addressed. Tel. +81-438-52-3933, Fax. +81-438-52-3934, E-mail: tabata{at}kazusa.or.jp

The nucleotide sequence of the entire chloroplast genome (150,519 bp) of a legume, Lotus japonicus, has been determined. The circular double-stranded DNA contains a pair of inverted repeats of 25,156 bp which are separated by a small and a large single copy region of 18,271 bp and 81,936 bp, respectively. A total of 84 predicted protein-coding genes including 7 genes duplicated in the inverted repeat regions, 4 ribosomal RNA genes and 37 tRNA genes (30 gene species) representing 20 amino acids species were assigned on the genome based on similarity to genes previously identified in other chloroplasts. All the predicted genes were conserved among dicot plants except that rpl22, a gene encoding chloroplast ribosomal protein CL22, was missing in L. japonicus. Inversion of a 51-kb segment spanning rbcL to rps16 (positions 5161–56,176)in the large single copy region was observed in the chloroplast genome of L. japonicus.

The sequence data and gene information are available on our World Wide Web database at http://www.kazusa.or.jp/en/plant/database.html.

Key words: Lotus japonicus; legume; chloroplast; genome sequencing


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