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DNA Research 2000 7(3):223-227; doi:10.1093/dnares/7.3.223
© 2000 by Kazusa DNA Research Institute
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Generation of 10,154 Expressed Sequence Tags from a Leafy Gametophyte of a Marine Red Alga, Porphyra yezoensis

Itoshi Nikaido1, Erika Asamizu2, Maiko Nakajima3, Yasukazu Nakamura2, Naotsune Saga1,3 and Satoshi Tabata2,*

1Graduate School of Marine Science and Technology, Tokai University Shimizu, Shizuoka 424-8610, Japan
2Kazusa DNA Research Institute Kisarazu, Chiba 292-0812, Japan
3Center for Advanced Technology, Tokai University Shimizu, Shizuoka 424-8610, Japan

* To whom correspondence should be addressed. Tel. +81-438-52-3933, Fax. +81-438-52-3934, E-mail: tabata{at}kazusa.or.jp

A total of 10,154 5'-end expressed sequence tags (EST) were established from the normalized and size-selected cDNA libraries of a marine red alga, Porphyra yezoensis. Among the ESTs, 2140 were unique species, and the remaining 8014 were grouped into 1127 species. Database search of the 3267 non-redundant ESTs by BLAST algorithm showed that the sequences of 1080 species (33.1%) have similarity to those of registered genes from various organisms including higher plants, mammals, yeasts, and cyanobacteria,while 2187 (66.9%) are novel. Codon usage analysis in the coding regions of 101 non-redundant EST groups showing significant similarity to known genes indicated the higher GC contents at the third position of codons (79.4%) than the first (62.2%) and the second position (45.0%), suggesting that the genome hasbeen exposed to high GC pressure during evolution. The sequence data of individual ESTs are available at the web site http://www.kazusa.or.jp/en/plant/porphyra/EST/.

Key words: Porphyra yezoensis; Rhodophyta; red alga; EST; codon usage


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