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DNA Research 2000 7(3):213-216; doi:10.1093/dnares/7.3.213
© 2000 by Kazusa DNA Research Institute
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Analysis of Expressed Sequence Tags of Flower Buds in Lotus japonicus

Makoto Endo1, Takao Kokubun1,2, Yoshihito Takahata1, Atsushi Higashitani3, Satoshi Tabata4 and Masao Watanabe1,*

1Laboratory of Plant Breeding, Faculty of Agriculture, Iwate University 3-18-8, Ueda, Morioka 020-8550, Japan
2Soma Agricultural High School 1-65, Mishima-machi, Haramachi 975-0012, Japan
3Institute of Genetic Ecology, Tohoku University 2-1-1, Katahira, Aoba-ku, Sendai 980-8577, Japan
4Kazusa DNA Research Institute 1532-3, Yana, Kisarazu 292-0812, Japan

* To whom correspondence should be addressed. Tel. +81-19-621-6152, Fax. +81-19-621-6177, E-mail: nabe{at}iwate-u.ac.jp

In order to study gene expression in a reproductive organ, we constructed a cDNA library of mature flower buds in Lotus japonicus, and characterized expressed sequence tags (ESTs) of 842 clones randomlyselected. The EST sequences were clustered into 718 non-redundant groups. From BLAST and FASTA search analyses of both protein and DNA databases, 58.5% of the EST groups showed significant sequence similarities to known genes. Several genes encoding these EST clones were identified as pollen-specific genes, such as pectin methylesterase, ascorbate oxidase, and polygalacturonase, and as homologous genes involved in pollen-pistil interaction. Comparison of these EST sequences with those derived from the whole plant of L. japonicus, revealed that 64.8% of EST sequences from the flower buds were not found in EST sequences of the whole plant. Taken together, the EST data from flower buds generated in this study is useful in dissecting gene expression in floral organ of L. japonicus.

Key words: Legume; Lotus japonicus; ESTs; flower bud; pollen-specific gene


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