Skip Navigation

DNA Research 2000 7(3):195-206; doi:10.1093/dnares/7.3.195
© 2000 by Kazusa DNA Research Institute
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (12)
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Yatsuki, H.
Right arrow Articles by Mukai, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yatsuki, H.
Right arrow Articles by Mukai, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Sequence-based Structural Features between Kvlqt1 and Tapa1 on Mouse Chromosome 7F4/F5 Corresponding to the Beckwith-Wiedemann Syndrome Region on Human 11p15.5 : long-stretches of Unusually Well Conserved Intronic Sequences of Kvlqt1 between Mouse and Human

Hitomi Yatsuki1, Hidemi Watanabe2, Masahira Hattori2, Keiichiro Joh1, Hidenobu Soejima1, Hiroshi Komoda1, Zhenghan Xin1, Xike Zhu1, Ken Higashimoto1, Masayo Nishimura1, Shinobu Kuratomi1, Hiroyuki Sasaki3, Yoshiyuki Sakaki2,4 and Tsunehiro Mukai1,*

1Department of Biochemistry, Saga Medical School 5-1-1 Nabeshima, Saga, Saga 849-8501, Japan
2RIKEN Genomic Science Center (GSC), Human Genome Research Group, c/o Kitasato University 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan
3Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics and Department of Genetics, Graduate University for Advanced Studies 1-111 Yata, Mishima, Shizuoka 411-8540, Japan
4Human Genome Center, Institute of Medical Science, University of Tokyo 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan

* To whom correspondence should be addressed. Tel. +81-952-34-2260, Fax. +81-952-34-2067, E-mail: mukait{at}post.sagamed.ac.jp

Mouse chromosome 7F4/F5 is a syntenic locus of human 11p15.5 in which many imprinted genes are clustered. Transmission of aberrant human 11p15.5 or duplicated 11p causes Beckwith-Wiedemann syndrome (BWS) depending on which parent the chromosome is derived from. To analyze a syntenic mouse locus corresponding to human 11p15.5, mouse BAC contigs were constructed between Nap2 and Tapa1, in which 390 kb was sequenced between Kvlqt1 and Tapa1. An unexpected finding was that of highlyconserved intronic sequences of Kvlqt1 between mouse and human, and their homologies came up to at least 160 kb because the length of this gene extended to 350 kb, suggesting the possibilityof some functional constraint due to transcriptional and/or post-transcriptional regulation of this region. Manyexpressed sequence tags (ESTs) were mapped on this locus. Three genes, Lit1 (Kvlqt1-AS), Mtr1 and Tssc4, were identified and characterized. Lit1 is an antisense-transcript of Kvlqt1 and paternally expressed and maternally methylated throughout the developmental stage. The position where Lit1 exists corresponded to a highly conserved region between mouse and human. This transcript extends at least 60 kb from downstream to upstream of exon 10 in Kvlqt1. Tssc4 and Mtr1 carried putative open reading frames but neither was imprinted. Further characterization of this locus based on the sequence comparison between mouse and human will contribute valuable information towards resolving the mechanism of the occurrence of BWS and the associated childhood tumor.

Key words: genomic imprinting; mouse chromosome 7F4/F5; Lit1 (Kvlqt1-AS); Mtr1; Tssc4


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
M. Rodova, M. R. Islam, K. R. Peterson, and J. P. Calvet
Remarkable Sequence Conservation of the Last Intron in the PKD1 Gene
Mol. Biol. Evol., October 1, 2003; 20(10): 1669 - 1674.
[Abstract] [Full Text]

Home page
 Genome ResHome page
H. Yatsuki, K. Joh, K. Higashimoto, H. Soejima, Y. Arai, Y. Wang, I. Hatada, Y. Obata, H. Morisaki, Z. Zhang, et al.
Domain Regulation of Imprinting Cluster in Kip2/Lit1 Subdomain on Mouse Chromosome 7F4/F5: Large-Scale DNA Methylation Analysis Reveals That DMR-Lit1 Is a Putative Imprinting Control Region
Genome Res., December 1, 2002; 12(12): 1860 - 1870.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
J. S. Mattick and M. J. Gagen
Review ArticleThe Evolution of Controlled Multitasked Gene Networks: The Role of Introns and Other Noncoding RNAs in the Development of Complex Organisms
Mol. Biol. Evol., September 1, 2001; 18(9): 1611 - 1630.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
S.-i. Horike, K. Mitsuya, M. Meguro, N. Kotobuki, A. Kashiwagi, T. Notsu, T. C. Schulz, Y. Shirayoshi, and M. Oshimura
Targeted disruption of the human LIT1 locus defines a putative imprinting control element playing an essential role in Beckwith-Wiedemann syndrome
Hum. Mol. Genet., September 1, 2000; 9(14): 2075 - 2083.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.