© 1998 by Kazusa DNA Research Institute
The Dictyostelium Developmental cDNA Project: Generation and Analysis of Expressed Sequence Tags from the First-Finger Stage of Development
1Institute of Biological Sciences, Univ. Tsukuba, Tsukuba, Ibaraki 305-8572, Japan
2Division of Biological Science, Graduate School of Sciences, Hokkaido Univ. Sapporo, Hokkaido 060-0810, Japan
3DNA Information & Stock Center, National Institute of Agrobiological Resources Tsukuba, Ibaraki 305-8602, Japan
4Research Institute of Food Science, Kinki Univ. Nara, Nara 631-8505, Japan
5Institute for Virus Research, Kyoto Univ. Kyoto, Kyoto 606-8507, Japan
6Faculty of Engineering, Toyama Univ. Toyama, Toyama 930-8555, Japan
7Department of Anatomy and Physiology, University of Dundee Dundee DD1 5EH, UK
8Department of Biology, Graduate School of Science, Osaka Univ. Toyonaka, Osaka 560-0043, Japan
9Novartis Foundation for the Promotion of Science Takarazuka, Hyogo 665-0042, Japan
* To whom correspondence should be addressed. Tel. +81-298-53-4666, Fax. +81-298-53-6614
In an effort to identify and characterize genes expressed during multicellular development in Dictyostelium, we have undertaken a cDNA sequencing project. Using size-fractionated subsets of cDNA from the first finger stage, two sets of gridded libraries were constructed for cDNA sequencing. One, library S, consisting of 9984 clones, carries relatively short inserts, and the other, library L, which consists of 8448 clones, has longer inserts. We sequenced all the selected clones in library S from their 3'-ends, and this generated 3093 non-redundant, expressed sequence tags (ESTs). Among them, 246 ESTs hit known Dictyostelium genes and 910 showed significant similarity to genes of Dictyostelium and other organisms. For library L, 1132 clones were randomly sequenced and 471 non-redundant ESTs were obtained. In combination, the ESTs from the two libraries represent approximately 40% of genes expressed in late development, assuming that the non-redundant ESTs correspond to independent genes. They will provide a useful resource for investigating the genetic networks that regulate multicellular development of this organism.
Key words: Dictyostelium discoideum; cDNA project; ESTs; multicellular development
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