© 1998 by Kazusa DNA Research Institute
Complete Nucleotide Sequences of 93-kb and 3.3-kb Plasmids of an Enterohemorrhagic Escherichia coli O157:H7 Derived from Sakai Outbreak
1Department of Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University Suita, Osaka 565, Japan
2Human Genome Center, Institute of Medical Science, The University of Tokyo Shirokanedai, Minato-ku, Tokyo 108, Japan
3Genome Information Research Center, Osaka University Suita, Osaka 565, Japan
4Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University Osaka 565, Japan
5Institute of Molecular and Cellular Biosciences, The University of Tokyo Bunkyo-ku, Tokyo 113, Japan
6Department of Bacteriology, School of Medicine, Shinshu University Matsumoto 390, Japan
7Graduate School of Genetic Resources Technology, Kyusyu University Hakozaki, Fukuoka 812, Japan
* To whom correspondence should be addressed. Tel. +81-6-879-8317, Fax. +81-6-879-8320, E-mail: shinagaw{at}biken.osakau.ac.jp
Enterohemorrhagic Escherichia coli (EHEC) O157:H7, derived from an outbreak in Sakai city, Japan in 1996, possesses two kinds of plasmids: a 93-kb plasmid termed pO157, found in clinical EHEC isolates world-wide and a 3.3-kb plasmid termed pOSAK1, prevalent in EHEC strains isolated in Japan. Complete nucleotide sequences of both plasmids have been determined, and the putative functions of the encoded proteins and the cis-acting DNA sequences have been analyzed. pO157 shares strikingly similar genes and DNA sequences with F-factor and the transmissible drug-resistant plasmid R100 for DNA replication, copy number control, plasmid segregation, conjugative functions and stable maintenance in the host, although it is defective in DNA transfer by conjugation due to the truncation and deletion of the required genes and DNA sequences. In addition, it encodes several proteins implicated in EHEC pathogenicity such as an EHEC hemolysin (HlyA), a catalase-peroxidase (KatP), a serine protease (EspP) and type II secretion system. pOSAK1 possesses a ColE1-like replication system, and the DNA sequence is extremely similar to that of a drug-resistant plasmid, NTP16, derived from Salmonella typhimurium except that it lacks drug resistance transposons.
Key words: Enterohemorrhagic Escherichia coli; F-factor; nucleotide sequence; pathogenicity; plasmid; pO157; pOSAK1
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