© 1997 by Kazusa DNA Research Institute
Sequence Analysis of a 685-kb Genomic Region on Chromosome 3p22–p21.3 That Is Homozygously Deleted in a Lung Carcinoma Cell Line*
1Laboratory of Molecular Medicine, Institute of Medical Science, the University of Tokyo 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan
2Department of Human Genome Analysis, the Cancer Chemotherapy Center, Japanese Foundation for Cancer Research 1-37-1 Kami-ikebukuro, Toshima-ku, Tokyo 170, Japan
3Dept of Surgery II, Kumamoto University Medical School Kumamoto, Japan
* To whom correspondence should be addressed. Tel. 81-3-5449-5372, Fax. 81-3-5449-5433
Frequent chromosomal aberrations and/or losses of heterozygosity involving the short arm of chromosome 3 in carcinomas of the lung, kidney and other tissues imply that multiple putative tumor suppressor genes may be present on this chromosomal arm. To search for one of these genes, we determined DNA sequences in the genomic region at 3p22–21.3 where we had previously detected a homozygous deletion in a lung cancer cell line. The DNA sequence results of an about 685-kb region indicated that the size of the homozygously deleted segment was 638,489 bp, in which we identified only four genes including the integrin 
RLC and the trans-Golgi p230 genes, both reported previously. The predicted amino acid sequences of one of the two novel genes showed high homology to villin, a human cytoskeleton protein; those of the other gene, termed HYA22, revealed significant homology to YA22, a hypothetical protein predicted from DNA sequences of Schizosaccharomyces pombe. The computer programs HEXON or GRAIL were able to predict three-fourths of the exons; the smallest exon predicted by either program was 46 base pairs. Repetitive sequences contained in the genomic region included 151 copies of the Alu sequence (1 copy/every 4.5 kb), 19 copies of the L1 sequence (1 copy/every 36 kb) , and 10 copies of the THE sequence.
Key words: homozygous deletion; DNA sequencing; villin-like protein
* Communicated by Yoshiyuki Sakaki
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. P. George, Y. Wang, S. Mathew, K. Srinivasan, and S. Khurana Dimerization and Actin-bundling Properties of Villin and Its Role in the Assembly of Epithelial Cell Brush Borders J. Biol. Chem., September 7, 2007; 282(36): 26528 - 26541. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kim, M. S. Gentry, T. E. Harris, S. E. Wiley, J. C. Lawrence Jr, and J. E. Dixon A conserved phosphatase cascade that regulates nuclear membrane biogenesis PNAS, April 17, 2007; 104(16): 6596 - 6601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sasai, J. T. Romer, H. Kimura, D. E. Eberhart, D. S. Rice, and T. Curran Medulloblastomas Derived from Cxcr6 Mutant Mice Respond to Treatment with a Smoothened Inhibitor Cancer Res., April 15, 2007; 67(8): 3871 - 3877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Claussen, K. Horvay, and T. Pieler Evidence for overlapping, but not identical, protein machineries operating in vegetal RNA localization along early and late pathways in Xenopus oocytes Development, September 1, 2004; 131(17): 4263 - 4273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kazemi, S. Papadopoulou, S. Li, Q. Su, S. Wang, A. Yoshimura, G. Matlashewski, T. E. Dever, and A. E. Koromilas Control of {alpha} Subunit of Eukaryotic Translation Initiation Factor 2 (eIF2{alpha}) Phosphorylation by the Human Papillomavirus Type 18 E6 Oncoprotein: Implications for eIF2{alpha}-Dependent Gene Expression and Cell Death Mol. Cell. Biol., April 15, 2004; 24(8): 3415 - 3429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. I. Kashuba, J. Li, F. Wang, V. N. Senchenko, A. Protopopov, A. Malyukova, A. S. Kutsenko, E. Kadyrova, V. I. Zabarovska, O. V. Muravenko, et al. RBSP3 (HYA22) is a tumor suppressor gene implicated in major epithelial malignancies PNAS, April 6, 2004; 101(14): 4906 - 4911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yeo, P. S. Lin, M. E. Dahmus, and G. N. Gill A Novel RNA Polymerase II C-terminal Domain Phosphatase That Preferentially Dephosphorylates Serine 5 J. Biol. Chem., July 3, 2003; 278(28): 26078 - 26085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Li, A. Protopopov, F. Wang, V. Senchenko, V. Petushkov, O. Vorontsova, L. Petrenko, V. Zabarovska, O. Muravenko, E. Braga, et al. NotI subtraction and NotI-specific microarrays to detect copy number and methylation changes in whole genomes PNAS, August 6, 2002; 99(16): 10724 - 10729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Matsushima-Nishiu, M. Unoki, K. Ono, T. Tsunoda, T. Minaguchi, H. Kuramoto, M. Nishida, T. Satoh, T. Tanaka, and Y. Nakamura Growth and Gene Expression Profile Analyses of Endometrial Cancer Cells Expressing Exogenous PTEN Cancer Res., May 1, 2001; 61(9): 3741 - 3749. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. W. van Nues and J. D. Beggs Functional Contacts With a Range of Splicing Proteins Suggest a Central Role for Brr2p in the Dynamic Control of the Order of Events in Spliceosomes of Saccharomyces cerevisiae Genetics, April 1, 2001; 157(4): 1451 - 1467. [Abstract] [Full Text]
|
|
|
|
|
|
M. I. Lerman and J. D. Minna The 630-kb Lung Cancer Homozygous Deletion Region on Human Chromosome 3p21.3: Identification and Evaluation of the Resident Candidate Tumor Suppressor Genes Cancer Res., November 1, 2000; 60(21): 6116 - 6133. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Kohno and J. Yokota1 How many tumor suppressor genes are involved in human lung carcinogenesis? Carcinogenesis, August 1, 1999; 20(8): 1403 - 1410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Daigo, T. Nishiwaki, T. Kawasoe, M. Tamari, E. Tsuchiya, and Y. Nakamura Molecular Cloning of a Candidate Tumor Suppressor Gene, DLC1, from Chromosome 3p21.3 Cancer Res., April 1, 1999; 59(8): 1966 - 1972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sakabe, T. Shinomiya, T. Mori, Y. Ariyama, Y. Fukuda, T. Fujiwara, Y. Nakamura, and J. Inazawa Identification of a Novel Gene, MASL1 within an Amplicon at 8p23.1 Detected in Malignant Fibrous Histiocytomas by Comparative Genomic Hybridization Cancer Res., February 1, 1999; 59(3): 511 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Marks, M Arai, J. Bandura, and D. Kwiatkowski Advillin (p92): a new member of the gelsolin/villin family of actin regulatory proteins J. Cell Sci., January 8, 1998; 111(15): 2129 - 2136. [Abstract] [PDF]
|
|