Skip Navigation


DNA Research Advance Access originally published online on March 4, 2009
DNA Research 2009 16(2):115-129; doi:10.1093/dnares/dsp003
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
16/2/115    most recent
dsp003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Nakao, Y.
Right arrow Articles by Ashikari, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakao, Y.
Right arrow Articles by Ashikari, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Kazusa DNA Research Institute.
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Genome Sequence of the Lager Brewing Yeast, an Interspecies Hybrid

Yoshihiro Nakao1,*, Takeshi Kanamori1, Takehiko Itoh2, Yukiko Kodama1, Sandra Rainieri1 {dagger}, Norihisa Nakamura1, Tomoko Shimonaga1, Masahira Hattori3 and Toshihiko Ashikari4

1 R&D Planning Division, Suntory Limited, 1-1-1, Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, Japan
2 Mitsubishi Research Institute, Advanced Science and Technology Group, 3-6 Otemachi 2-chome Chiyoda-ku, Tokyo 100-8141, Japan
3 Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa-no-ha 5-1-5, Kashiwa, Chiba 277-8561, Japan
4 Florigene Pty, Ltd, 1 Park Drive, Bundoora, VIC 3083, Australia

Received 30 September 2008 ; accepted 7 February 2009.

This work presents the genome sequencing of the lager brewing yeast (Saccharomyces pastorianus) Weihenstephan 34/70, a strain widely used in lager beer brewing. The 25 Mb genome comprises two nuclear sub-genomes originating from Saccharomyces cerevisiae and Saccharomyces bayanus and one circular mitochondrial genome originating from S. bayanus. Thirty-six different types of chromosomes were found including eight chromosomes with translocations between the two sub-genomes, whose breakpoints are within the orthologous open reading frames. Several gene loci responsible for typical lager brewing yeast characteristics such as maltotriose uptake and sulfite production have been increased in number by chromosomal rearrangements. Despite an overall high degree of conservation of the synteny with S. cerevisiae and S. bayanus, the syntenies were not well conserved in the sub-telomeric regions that contain lager brewing yeast characteristic and specific genes. Deletion of larger chromosomal regions, a massive unilateral decrease of the ribosomal DNA cluster and bilateral truncations of over 60 genes reflect a post-hybridization evolution process. Truncations and deletions of less efficient maltose and maltotriose uptake genes may indicate the result of adaptation to brewing. The genome sequence of this interspecies hybrid yeast provides a new tool for better understanding of lager brewing yeast behavior in industrial beer production.

Key words: Saccharomyces pastorianus; beer; genome; interspecies hybrid; larger yeast

* To whom correspondence should be addressed. Tel. +81 75-962-2365. Fax. +81 75-962-3791. E-mail: yoshihiro_nakao{at}suntory.co.jp

{dagger} Present address: AZTI-Tecnalia, Food Research Division, Parque Tecnológico de Bizkaia, Astondo Bidea 609, 48160, Derio, Spain


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
R. J. S. Baerends, J.-L. Qiu, S. Rasmussen, H. B. Nielsen, and A. Brandt
Impaired Uptake and/or Utilization of Leucine by Saccharomyces cerevisiae Is Suppressed by the SPT15-300 Allele of the TATA-Binding Protein Gene
Appl. Envir. Microbiol., October 1, 2009; 75(19): 6055 - 6061.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
J. Usher and U. Bond
Recombination between Homoeologous Chromosomes of Lager Yeasts Leads to Loss of Function of the Hybrid GPH1 Gene
Appl. Envir. Microbiol., July 1, 2009; 75(13): 4573 - 4579.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.