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DNA Research Advance Access originally published online on April 4, 2008
DNA Research 2008 15(3):115-122; doi:10.1093/dnares/dsn005
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© The Author 2008. Kazusa DNA Research Institute
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Transcriptome Analysis of a cDNA Library from Adult Human Epididymis

Jian-Yuan Li1,*, Hai-Yan Wang1, Jie Liu1, Qiang Liu2, Jin-Song Zhang2, Feng-Chun Wan1, Fu-Jun Liu1, Shao-Hua Jin1 and Yong-Lian Zhang2,*

1 Shandong Research Center of Stem Cell Engineering/Centre Laboratory, Yu-Huang-Ding Hospital, Yan-Tai, Shan-Dong Province, Shandong 264000, People's Republic of China
2 Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, People's Republic of China

Received 28 June 2007 ; accepted 5 March 2008.

Mammalian Gene Collection (MGC) verified over 9000 human full-ORF genes and FLJ Program reported 21 243 cDNAs of which 14 409 were unique ones and 5416 seemed to be protein-coding. The pity is that epididymis cDNA library was missing in their sequencing target list. Epididymis is a very important male accessory sex organ for sperm maturation and storage. Fully differentiated spermatozoa left from testis acquire their motility and capacity for fertilization via interactions with the epididymal epithelium duct lumen during passage through this convoluted duct. Here, we report that 20 000 clones from a healthy male epididymis cDNA library have been sequenced. The sequencing data provided 8234 known sequences and 650 unknown cDNA fragments. Hundred and six of 650 unknown cDNA clone inserts were randomly selected for fully sequencing. There were 25 unknown unique sequences and 19 released but unreported sequences came out. By northern blot analysis, four sequences randomly selected from the 19 released sequences with no known function showed positive mRNA signals in epididymis and testis. The signals for three of six from those unknown group showed as epididymis abundant in a region-specific manner but not in the testis and other tissues tested. All the sequencing data will be available on the website www.sdscli.com.

Key words: human epididymis cDNA library; transcriptomes for human epididymis; sperm maturation

* To whom correspondence should be addressed. Tel. + 86 21-54921263. Fax. + 86 21-54921011. E-mail: sdscli{at}126.com (J-Y.L.), ylzhang{at}sibs.ac.cn (Y-L.Z.)

Edited by Osamu Ohara


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