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DNA Research 2008 15(1):49-62; doi:10.1093/dnares/dsm029
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© The Author 2008. Kazusa DNA Research Institute
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Revolver is a New Class of Transposon-like Gene Composing the Triticeae Genome

Motonori Tomita*, Kasumi Shinohara and Mayu Morimoto

Molecular Genetics Laboratory, Faculty of Agriculture, Tottori University, 101, Koyama-minami 4-chome, Tottori City, Tottori 680-8553, Japan

Received 22 May 2007 ; accepted 5 December 2007.

Revolver discovered in the Triticeae plant is a novel class of transposon-like gene and a major component of the large cereal genome. An 89 bp segment of Revolver that is enriched in the genome of rye was isolated by deleting the DNA sequences common to rye and wheat. The entire structure of Revolver was determined by using rye genomic clones, which were screened by the 89 bp probe. Revolver consists of 2929—3041 bp with an inverted repeated sequence on each end and is dispersed through all seven chromosomes of the rye genome. Revolver is transcriptionally active, and the isolated full-length cDNA (726 bp) reveals that Revolver harbors a single gene consisting of three exons (342, 88, and 296 bp) and two introns (750 and 1237 bp), and encodes 139 amino acid residues of protein, which shows similarity to some transcriptional regulators. Revolver variants ranging from 2665 to 4269 bp, in which 5' regions were destructed, indicate structural diversities around the first exon. Revolver does not share identity with any known class I or class II autonomous transposable elements of any living species. DNA blot analysis of Triticeae plants shows that Revolver has existed since the diploid progenitor of wheat, and has been amplified or lost in several species during the evolution of the Triticeae.

Key words: transposon-like gene; transcript; genomic component; structural and quantitative divergence

* To whom correspondence should be addressed. Tel. +81-857-31-5351. Fax. +81-857-31-5351. E-mail: tomita{at}muses.tottori-u.ac.jp

Edited by Satoshi Tabata


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