Isolation and Expression Profiling of Genes Upregulated in Bone Marrow-Derived Mononuclear Cells of Rheumatoid Arthritis Patients
1 Center of Arthroplasty, Kyowakai Hospital Suita, Japan
2 Yukioka Hospital Osaka, Japan
3 Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University 3-1 Yamadaoka, Suita, Osaka 562-0031, Japan
4 Innovation Plaza Osaka Izumi, Japan
5 Department of Rheumatology, NHO Osaka-Minami Medical Center Kawachinagano, Japan
6 Hoshigaoka Kosei-Nenkin Hospital Hirakata, Japan
7 Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital 18-1 Sakura-dai, Sagamihara, Kanagawa 228-8522, Japan
We have comprehensively identified the genes whose expressions are augmented in bone marrow-derived mononuclear cells (BMMC) from patients with Rheumatoid Arthritis (RA) as compared with BMMCs from Osteoarthritis (OA) patients, and named them AURA after augmented in RA. Both stepwise subtractive hybridization and microarray analyses were used to identify AURA genes, which were confirmed by northern blot analysis and/or reverse transcription polymerase chain reaction (RT–PCR). We also assessed their expression levels in individual patients by quantitative real-time RT–PCR. Of 103 AURA genes we have identified, the mRNA levels of the following 10 genes, which are somehow related to immune responses, were increased in many of the RA patients: AREG (=AURA9), FK506-binding protein 5 (FKBP5 = AURA45), C-type lectin superfamily member 9 (CLECSF9 = AURA24), tyrosylprotein sulfotransferase 1 (TPST1 = AURA52), lymphocyte G0/G1 switch gene (G0S2 = AURA8), chemokine receptor 4 (CXCR4 = AURA86), nuclear factor-kappa B (NF-
B = AURA25) and two genes of unknown function (FLJ11106 = AURA1, BC022398
[GenBank]
= AURA2 and XM_058513 = AURA17). Since AREG was most significantly increased in many of the RA patients, we subjected it to further analysis and found that AREG-epidermal growth factor receptor signaling is highly activated in synovial cells isolated from RA patients, but not in OA synoviocytes. We propose that the expression profiling of these AURA genes may improve our understanding of the pathogenesis of RA.
Key words: stepwise subtraction; microarray; RA; OA; amphiregulin; synoviolin
*To whom correspondence should be addressed. Tel. +81-6-6875-3980, Fax. +81-6-6875-5192, E-mail: snj-0212{at}biken.osaka-u.ac.jp
Communicated by Mitsuo Oshimura
These authors contributed equally to this work.
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