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DNA Research Advance Access originally published online on January 11, 2006
DNA Research 2005 12(5):379-387; doi:10.1093/dnares/dsi014
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© The Author 2006. Kazusa DNA Research Institute
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

InCeP: Intracellular Pathway Based on mKIAA Protein–Protein Interactions

Masatoshi Murakami1, Kiyo Shimada1, Makoto Kawai1 and Hisashi Koga1,2,*

1Chiba Industry Advancement Center 2-6 Nakase, Mihama-ku, Chiba 261-7126, Japan
2Kazusa DNA Research Institute 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan

Since December 2001, we have been conducting a project to isolate and determine entire sequences of mouse KIAA cDNA clones, which encode polypeptides corresponding to human KIAA proteins. The ultimate goal of this project has been elucidation of the functions of KIAA proteins. To address this issue, we have been generating ‘libraries’ of antibodies against mKIAA proteins. We have, to date, already generated >800 antibodies. Using our ‘libraries’ of antibodies, we are now identifying endogenous mKIAA protein–protein interactions. In the present study, novel interactions were identified by MS/MS analysis following immunoprecipitation with anti-mKIAA antibodies. The interactions with biologically known molecules should enable us to predict the function of mKIAA/KIAA proteins, including hypothetical proteins identified in our cDNA project. These interactions are subsequently used for construction of an intracellular pathway related to the mKIAA protein, and the pathway is distributed through the InCeP (IntraCellular Pathway based on mKIAA protein–protein interactions) database. Users can freely access the InCeP through the internet and download the graphical display as well as the curated information.

Key words: InCeP; mKIAA; protein–protein interactions; intracellular pathway

*To whom correspondence should be addressed. Tel. +81-438-52-3919, Fax. +81-438-52-3918, Email: hkoga{at}kazusa.or.jp

Communicated by Michio Oishi


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