© The Author 2005. Kazusa DNA Research Institute
Genome-wide Expression Analysis Reveals 100 Adrenal Gland-dependent Circadian Genes in the Mouse Liver
1Clock Cell Biology Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST) Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
2Graduate School of Life and Environmental Sciences, University of Tsukuba Tsukuba, Ibaraki 305-8502, Japan
3Computational Biology Research Center, National Institute of Advanced Industrial Science and Technology Aomi Frontier Bldg 17F, 2-43 Aomi, Koto-Ku, Tokyo 135-0064, Japan
4Transcriptome Research Center, National Institute of Radiological Sciences (NIRS) 4-9-1 Anagawa, Chiba, Chiba 263-8555, Japan
5Clinical Molecular Biology, Faculty of Pharmaceutical Sciences, Teikyo University 1091-1 Suarashi, Sagamiko, Tsukui, Kanagawa 199-0195, Japan
Recent progress in genome-wide expression analysis has identified hundreds of circadian genes not only in the suprachiasmatic nucleus (the mammalian master clock) but also in peripheral tissues, such as heart, liver and kidney of mammals. Glucocorticoid is thought to be a circadian time cue for mammalian peripheral clocks. To identify the genes of which the circadian expression is regulated by endogenous glucocorticoids, we performed DNA microarray analysis using hepatic RNA from adrenalectomized (ADX) and sham-operated mice. We identified 169 genes that fluctuated between day and night in the livers of the sham-operated mice. Among these, 100 lost circadian rhythmicity in ADX mice. These included the genes for key enzymes of liver metabolic functions, such as glucokinase, HMG-CoA reductase and glucose-6-phosphatase. The circadian expression of Lpin1, FKBP51 and S-adenosyl methionine decarboxylase was also abolished in the ADX mice. On the other hand, although the circadian expression of clock or clock-related genes, such as mPer2, DBP, E4BP4, mDec1, Usp2 and Wee1 remained almost totally intact in the liver of ADX mice, it was extremely damped in homozygous Clock mutant mice. The present findings suggested that one type of hepatic circadian genes in mice is transcriptionally regulated by core components of the circadian clock, such as CLOCK and BMAL1, and that the other depends on the adrenal gland.
Key words: circadian rhythm; glucocorticoids; Clock; DNA microarray; liver
*To whom correspondence should be addressed. Tel. +81-29-861-6053, Fax. +81-29-861-9499, E-mail: n.ishida{at}aist.go.jp
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Takeuchi and K. Reue Biochemistry, physiology, and genetics of GPAT, AGPAT, and lipin enzymes in triglyceride synthesis Am J Physiol Endocrinol Metab, June 1, 2009; 296(6): E1195 - E1209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Yang, Y.-K. J. Zhang, N. Esterly, C. D. Klaassen, and Y.-J. Y. Wan Gender Disparity of Hepatic Lipid Homoeostasis Regulated by the Circadian Clock J. Biochem., May 1, 2009; 145(5): 609 - 623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Girotti, M. S. Weinberg, and R. L. Spencer Diurnal expression of functional and clock-related genes throughout the rat HPA axis: system-wide shifts in response to a restricted feeding schedule Am J Physiol Endocrinol Metab, April 1, 2009; 296(4): E888 - E897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Dickmeis Glucocorticoids and the circadian clock J. Endocrinol., January 1, 2009; 200(1): 3 - 22. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. H. Son, S. Chung, H. K. Choe, H.-D. Kim, S.-M. Baik, H. Lee, H.-W. Lee, S. Choi, W. Sun, H. Kim, et al. Adrenal peripheral clock controls the autonomous circadian rhythm of glucocorticoid by causing rhythmic steroid production PNAS, December 30, 2008; 105(52): 20970 - 20975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Reue and D. N. Brindley Thematic Review Series: Glycerolipids. Multiple roles for lipins/phosphatidate phosphatase enzymes in lipid metabolism J. Lipid Res., December 1, 2008; 49(12): 2493 - 2503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Zhang, L. O'Loughlin, D. N. Brindley, and K. Reue Regulation of lipin-1 gene expression by glucocorticoids during adipogenesis J. Lipid Res., July 1, 2008; 49(7): 1519 - 1528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Sei, K. Oishi, S. Chikahisa, K. Kitaoka, E. Takeda, and N. Ishida Diurnal Amplitudes of Arterial Pressure and Heart Rate Are Dampened in Clock Mutant Mice and Adrenalectomized Mice Endocrinology, July 1, 2008; 149(7): 3576 - 3580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cailotto, C. van Heijningen, J. van der Vliet, G. van der Plasse, C. Habold, A. Kalsbeek, P. Pevet, and R. M. Buijs Daily Rhythms in Metabolic Liver Enzymes and Plasma Glucose Require a Balance in the Autonomic Output to the Liver Endocrinology, April 1, 2008; 149(4): 1914 - 1925. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Yang, R. R. Almon, D. C. DuBois, W. J. Jusko, and I. P. Androulakis Extracting Global System Dynamics of Corticosteroid Genomic Effects in Rat Liver J. Pharmacol. Exp. Ther., March 1, 2008; 324(3): 1243 - 1254. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. F. Reilly, A. M. Curtis, Y. Cheng, E. J. Westgate, R. D. Rudic, G. Paschos, J. Morris, M. Ouyang, S. A. Thomas, and G. A. FitzGerald Peripheral Circadian Clock Rhythmicity Is Retained in the Absence of Adrenergic Signaling Arterioscler. Thromb. Vasc. Biol., January 1, 2008; 28(1): 121 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Lemos, L. Goodspeed, L. Tonelli, M. P. Antoch, S. R. Ojeda, and H. F. Urbanski Evidence for Circadian Regulation of Activating Transcription Factor 5 But Not Tyrosine Hydroxylase by the Chromaffin Cell Clock Endocrinology, December 1, 2007; 148(12): 5811 - 5821. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Noshiro, E. Usui, T. Kawamoto, H. Kubo, K. Fujimoto, M. Furukawa, S. Honma, M. Makishima, K.-i. Honma, and Y. Kato Multiple Mechanisms Regulate Circadian Expression of the Gene for Cholesterol 7{alpha}-Hydroxylase (Cyp7a), a Key Enzyme in Hepatic Bile Acid Biosynthesis J Biol Rhythms, August 1, 2007; 22(4): 299 - 311. [Abstract] [PDF]
|
|
|
|
|
|
D. F. Reilly, E. J. Westgate, and G. A. FitzGerald Peripheral Circadian Clocks in the Vasculature Arterioscler. Thromb. Vasc. Biol., August 1, 2007; 27(8): 1694 - 1705. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ohno, Y. Onishi, and N. Ishida A novel E4BP4 element drives circadian expression of mPeriod2 Nucleic Acids Res., January 28, 2007; 35(2): 648 - 655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Yu, Z. Jian, S.-H. Shen, E. Purisima, and E. Wang Global analysis of microRNA target gene expression reveals that miRNA targets are lower expressed in mature mouse and Drosophila tissues than in the embryos Nucleic Acids Res., January 12, 2007; 35(1): 152 - 164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Oster, S. Damerow, R. A. Hut, and G. Eichele Transcriptional Profiling in the Adrenal Gland Reveals Circadian Regulation of Hormone Biosynthesis Genes and Nucleosome Assembly Genes J Biol Rhythms, October 1, 2006; 21(5): 350 - 361. [Abstract] [PDF]
|
|