Skip Navigation

DNA Research 2003 10(3):115-122; doi:10.1093/dnares/10.3.115
© 2003 by Kazusa DNA Research Institute
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Asamizu, E.
Right arrow Articles by Tabata, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Asamizu, E.
Right arrow Articles by Tabata, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Structural Analysis of a Lotus japonicus Genome. IV. Sequence Features and Mapping of Seventy-three TAC Clones Which Cover the 7.5 Mb Regions of the Genome

Erika Asamizu, Tomohiko Kato, Shusei Sato, Yasukazu Nakamura, Takakazu Kaneko and Satoshi Tabata*

Kazusa DNA Research Institute 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan

* To whom correspondence should be addressed. Tel. +81-438-52-3933, Fax. +81-438-52-3934, E-mail: tabata{at}kazusa.or.jp

Using the sequence information of expressed sequences tags (ESTs), cDNAs and genes from Lotus japonicus and other legumes, 73 TAC (transformation-competent arti.cial chromosomes) clones were selected from a genomic library of L. japonicus accession MG-20, and their nucleotide sequences were determined. The length of the DNA sequenced in this study was 7,455,959 bp, and the total length the DNA regions sequenced so far is 26,167,443 bp together with the nucleotide sequences of 183 TAC clones previously reported. By similarity searches against the sequences in protein and EST databases and prediction by computer programs, a total of 699 potential protein-encoding genes with known or predicted functions, 163 gene segments and 267 pseudogenes were assigned to the newly sequenced regions. Based on the nucleotide sequences of the clones, simple sequence repeat length polymorphism (SSLP) or derived cleaved amplified polymorphic sequence (dCAPS) markers were generated, and each clone was located onto the linkage map of two accessions of L. japonicus, Gifu B-129 and Miyakojima MG-20. The sequence data, gene information and mapping information are available through the World Wide Web at http://www.kazusa.or.jp/lotus/.

Key words: Lotus japonicus; genomic sequence; TAC genomic library; gene prediction; linkage mapping


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 DNA ResHome page
S. Sato, Y. Nakamura, T. Kaneko, E. Asamizu, T. Kato, M. Nakao, S. Sasamoto, A. Watanabe, A. Ono, K. Kawashima, et al.
Genome Structure of the Legume, Lotus japonicus
DNA Res, August 1, 2008; 15(4): 227 - 239.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Yoshida, R. Iwasaka, T. Kaneko, S. Sato, S. Tabata, and M. Sakuta
Functional Differentiation of Lotus japonicus TT2s, R2R3-MYB Transcription Factors Comprising a Multigene Family
Plant Cell Physiol., February 1, 2008; 49(2): 157 - 169.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Sato, Y. Nakamura, E. Asamizu, S. Isobe, and S. Tabata
Genome Sequencing and Genome Resources in Model Legumes
Plant Physiology, June 1, 2007; 144(2): 588 - 593.
[Full Text] [PDF]

Home page
 Genome ResHome page
K. Hanada, X. Zhang, J. O. Borevitz, W.-H. Li, and S.-H. Shiu
A large number of novel coding small open reading frames in the intergenic regions of the Arabidopsis thaliana genome are transcribed and/or under purifying selection
Genome Res., May 1, 2007; 17(5): 632 - 640.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Kumagai, T. Hakoyama, Y. Umehara, S. Sato, T. Kaneko, S. Tabata, and H. Kouchi
A Novel Ankyrin-Repeat Membrane Protein, IGN1, Is Required for Persistence of Nitrogen-Fixing Symbiosis in Root Nodules of Lotus japonicus
Plant Physiology, March 1, 2007; 143(3): 1293 - 1305.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. Holligan, X. Zhang, N. Jiang, E. J. Pritham, and S. R. Wessler
The Transposable Element Landscape of the Model Legume Lotus japonicus
Genetics, December 1, 2006; 174(4): 2215 - 2228.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
N. Shimada, R. Sasaki, S. Sato, T. Kaneko, S. Tabata, T. Aoki, and S.-i. Ayabe
A comprehensive analysis of six dihydroflavonol 4-reductases encoded by a gene cluster of the Lotus japonicus genome
J. Exp. Bot., September 1, 2005; 56(419): 2573 - 2585.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. D. Young, S. B. Cannon, S. Sato, D. Kim, D. R. Cook, C. D. Town, B. A. Roe, and S. Tabata
Sequencing the Genespaces of Medicago truncatula and Lotus japonicus
Plant Physiology, April 1, 2005; 137(4): 1174 - 1181.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Karas, J. Murray, M. Gorzelak, A. Smith, S. Sato, S. Tabata, and K. Szczyglowski
Invasion of Lotus japonicus root hairless 1 by Mesorhizobium loti Involves the Nodulation Factor-Dependent Induction of Root Hairs
Plant Physiology, April 1, 2005; 137(4): 1331 - 1344.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.